FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s10.352000000d7c1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s10.352000000d7c1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA70.3148897885739991No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG60.2699055330634278No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA60.2699055330634278No Hit
ACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTT40.1799370220422852No Hit
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT40.1799370220422852No Hit
CATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGC40.1799370220422852No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC40.1799370220422852No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGC30.1349527665317139No Hit
ACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGT30.1349527665317139No Hit
GTAGTATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGA30.1349527665317139No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT30.1349527665317139No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT30.1349527665317139No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC30.1349527665317139No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)