FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n02_r3zt14s1.352000000d7b0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n02_r3zt14s1.352000000d7b0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14279636
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1190090.8334176025215209No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA976620.6839249964074714No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT882380.6179289163953479No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA652800.4571545101009577No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT438930.30738178480179745No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC381310.2670306161865751No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC362280.2537039459549249No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG328060.22973974966868904No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA254860.17847793879339782No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT219850.15396050711656795No Hit
GAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAG192470.13478634889572816No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT173830.12173279486956111No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA167450.11726489386704256No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG161670.1132171716421903No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA161450.11306310609037933No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA142950.1001075937790011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC481850.044.9746976
GAGACGT449200.044.5966848
AGACGTG438800.042.320029
GGAGACG515650.040.8575447
AGGGAGA249050.038.387245
TGGGAGA192100.035.2303665
CAGGGAG154250.032.298324
TAGGGAG76250.029.523524
CGGGAGA129150.026.0356525
CCGATCT695000.025.9400634
TTCCGAT723900.025.1603372
TCCGATC735200.024.9157373
AGACGTT56050.024.0645899
CTTCCGA848450.022.4856381
CTAGGGA42550.022.4350033
GGGGAGA127750.021.8597935
CGATCTG404650.021.337525
ACGTGTG463300.020.87658110-11
TATGCTT101650.020.8380535
GACGTGT456200.020.68615710-11
Produced by FastQC (version 0.11.2)