FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s94.351000000d86cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s94.351000000d86cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12459737
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA718320.5765129713412089No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT604910.4854917884703345No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT534690.4291342586123607No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA515350.4136122616392304No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT461500.3703930508324534No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT311060.2496521395275037No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT162990.1308133550491475Illumina PCR Primer Index 7 (95% over 22bp)
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG153180.12293999464033632Illumina PCR Primer Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT345350.035.3750536
ATCTTTT342850.034.538527
CGATCTT477400.030.535155
TCTTTTT456300.025.9511998
CTTTTTT499100.023.6686699
CCGATCT1645200.021.0703014
TTCCGAT1671600.020.780242
TCCGATC1704150.020.3943963
CTTCCGA1737250.020.1819761
GATCTGG182200.017.7274446
CGATCTG613750.017.335735
GTCCTAA55500.016.4464511
CTAGGAC54100.016.3305683
GTCCTAG38750.016.3171351
GTATTAT28850.016.1489491
TAATACT59250.015.9532934
TATACTT32700.015.6877845
GTACTAT74550.015.6874151
GTATAGT41450.015.5983391
TCTAGGA60750.015.32488252
Produced by FastQC (version 0.11.2)