HG5M3BCX2_l02n01_r3zt14s93.351000000d8697.fastq.gz FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l02n01_r3zt14s93.351000000d8697.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2013
Sequences flagged as poor quality	0
Sequence length	101
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC	21	1.0432190760059614	Illumina PCR Primer Index 10 (96% over 25bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC	19	0.9438648782911079	RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC	18	0.8941877794336811	RNA PCR Primer, Index 11 (96% over 27bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA	10	0.49677098857426727	Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA	9	0.44709388971684055	Illumina PCR Primer Index 11 (100% over 29bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA	7	0.34773969200198707	Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA	6	0.29806259314456035	RNA PCR Primer, Index 17 (96% over 28bp)
CCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	5	0.24838549428713363	RNA PCR Primer, Index 17 (96% over 28bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT	4	0.19870839542970692	No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	4	0.19870839542970692	No Hit
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	4	0.19870839542970692	Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	3	0.14903129657228018	No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA	3	0.14903129657228018	TruSeq Adapter, Index 3 (96% over 28bp)
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT	3	0.14903129657228018	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.14903129657228018	No Hit
GTACTGATGCTGCATATGCCTGTGTAACGCCCACGAGACCCTAAGACATC	3	0.14903129657228018	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.14903129657228018	No Hit
CATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGC	3	0.14903129657228018	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers