HG5M3BCX2_l02n01_r3zt14s91.351000000d8660.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l02n01_r3zt14s91.351000000d8660.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1257
Sequences flagged as poor quality	0
Sequence length	101
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC	41	3.261734287987271	Illumina Paired End PCR Primer 2 (96% over 26bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA	16	1.2728719172633254	RNA PCR Primer, Index 42 (96% over 28bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA	6	0.47732696897374705	TruSeq Adapter, Index 5 (96% over 28bp)
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC	5	0.3977724741447892	Illumina PCR Primer Index 5 (96% over 25bp)
GATCGGAAGAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGC	5	0.3977724741447892	RNA PCR Primer, Index 1 (95% over 23bp)
GATCGGAAGAGCCCACGAGACACTGAGCGAGCTCGTATGCCGTCTTCTGC	3	0.23866348448687352	RNA PCR Primer, Index 1 (95% over 23bp)
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.23866348448687352	Illumina Paired End PCR Primer 2 (96% over 29bp)
GCACCCCTGTCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	3	0.23866348448687352	No Hit
ATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT	3	0.23866348448687352	RNA PCR Primer, Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.23866348448687352	No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	3	0.23866348448687352	No Hit
GCACCCCTGTCTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	3	0.23866348448687352	No Hit
GCACCCCGGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	2	0.15910898965791567	No Hit
GCACCCCTGCCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	2	0.15910898965791567	No Hit
CACCCCAGGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT	2	0.15910898965791567	No Hit
CCCCAGAGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTAGCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
GAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGCTTGAAAAA	2	0.15910898965791567	RNA PCR Primer, Index 33 (96% over 27bp)
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC	2	0.15910898965791567	No Hit
CCCCGCCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC	2	0.15910898965791567	No Hit
CCCCTGGCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
TTTCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC	2	0.15910898965791567	Illumina Paired End PCR Primer 2 (96% over 26bp)
CCCCGGGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCCGTTTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTGGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CACCCCTTGGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATG	2	0.15910898965791567	No Hit
GCACCCCGGCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	2	0.15910898965791567	No Hit
CCCTCGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC	2	0.15910898965791567	No Hit
AAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT	2	0.15910898965791567	TruSeq Adapter, Index 5 (95% over 23bp)
CCCCGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC	2	0.15910898965791567	No Hit
CCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2	0.15910898965791567	Illumina Paired End PCR Primer 2 (96% over 29bp)
CCCCTGCGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
AAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2	0.15910898965791567	Illumina Paired End PCR Primer 2 (95% over 23bp)
CCCCTCGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCCGA	2	0.15910898965791567	No Hit
GCACCCCCGTCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	2	0.15910898965791567	No Hit
CCCCATGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTGGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTTGGGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTTGAGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCTGGGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCCGCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit
CCCCGTTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	2	0.15910898965791567	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAGCC	65	5.7767247E-6	25.576923	16-17
GATCGGA	65	5.7767247E-6	25.576923	10-11
GGAAGAG	65	5.7767247E-6	25.576923	14-15
TCGGAAG	70	1.02876475E-5	23.75	12-13
AGATCGG	60	0.003580101	19.791668	10-11
GCCCACG	85	4.638167E-5	19.558823	20-21
GAGCCCA	85	4.638167E-5	19.558823	18-19
CACTGAG	85	4.638167E-5	19.558823	30-31
GACACTG	85	4.638167E-5	19.558823	28-29
GCTTGAA	85	4.638167E-5	19.558823	58-59
TTGAAAA	85	4.638167E-5	19.558823	60-61
GAGACAC	85	4.638167E-5	19.558823	26-27
CTGAGCG	90	7.212522E-5	18.472221	32-33
GAAAAAA	90	7.212522E-5	18.472221	62-63
TTCTGCT	90	7.212522E-5	18.472221	54-55
ACGAGAC	90	7.212522E-5	18.472221	24-25
ATGCCGT	90	7.212522E-5	18.472221	46-47
CTGCTTG	90	7.212522E-5	18.472221	56-57
GCCGTCT	90	7.212522E-5	18.472221	48-49
TCTTCTG	90	7.212522E-5	18.472221	52-53

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content