FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s90.351000000d8654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s90.351000000d8654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC494.757281553398058RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC121.1650485436893203Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA111.0679611650485437Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA80.7766990291262136RNA PCR Primer, Index 17 (96% over 28bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.48543689320388345No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.2912621359223301Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA30.2912621359223301TruSeq Adapter, Index 3 (96% over 28bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2912621359223301No Hit
AAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC30.2912621359223301No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG30.2912621359223301No Hit
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT30.2912621359223301No Hit
GTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACT20.1941747572815534No Hit
GGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTG20.1941747572815534No Hit
CCCCATGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.1941747572815534No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT20.1941747572815534No Hit
TTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCG20.1941747572815534No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT20.1941747572815534No Hit
GCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAA20.1941747572815534No Hit
CCCTGGGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC20.1941747572815534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1941747572815534No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1941747572815534No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACAGCTCGTATGCCGTCTTCTGC20.1941747572815534Illumina PCR Primer Index 11 (95% over 23bp)
CCCCTGGCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.1941747572815534No Hit
CCCCGGGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)