HG5M3BCX2_l02n01_r3zt14s9.351000000d7bf2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l02n01_r3zt14s9.351000000d7bf2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1989
Sequences flagged as poor quality	0
Sequence length	101
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC	57	2.865761689291101	Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA	13	0.6535947712418301	Illumina PCR Primer Index 10 (96% over 28bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA	9	0.4524886877828055	No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA	6	0.301659125188537	Illumina PCR Primer Index 10 (96% over 28bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	6	0.301659125188537	Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC	5	0.2513826043237808	RNA PCR Primer, Index 10 (96% over 25bp)
TCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT	4	0.20110608345902461	Illumina PCR Primer Index 10 (96% over 27bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	3	0.1508295625942685	No Hit
TTCCAGGACTTACGCTCTTGATGCGGGAAACGGCGTCGGCCGGATTATCC	3	0.1508295625942685	No Hit
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT	3	0.1508295625942685	No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT	3	0.1508295625942685	No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA	3	0.1508295625942685	No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC	3	0.1508295625942685	No Hit
GGTTGATGTGGCGCAACAGCTTCACGCCGCAGAGCAGCATGGCAGTGGGA	3	0.1508295625942685	No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGC	3	0.1508295625942685	RNA PCR Primer, Index 12 (95% over 23bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3	0.1508295625942685	No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG	3	0.1508295625942685	No Hit
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA	3	0.1508295625942685	Illumina PCR Primer Index 10 (96% over 28bp)
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT	3	0.1508295625942685	No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA	3	0.1508295625942685	No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT	3	0.1508295625942685	No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACGAGACA	3	0.1508295625942685	No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC	3	0.1508295625942685	No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT	3	0.1508295625942685	No Hit
CGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG	3	0.1508295625942685	Illumina PCR Primer Index 10 (96% over 28bp)
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	3	0.1508295625942685	No Hit
ATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGT	3	0.1508295625942685	No Hit
TGGTAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	2	0.10055304172951231	No Hit
GATCTGGGATGGGAAGAGCACCCCGGTGAGATCGGAAGAGCCCACGAGAC	2	0.10055304172951231	No Hit
ACACGGTCTCGCGCATCAGGTCCGAATGCATCGCCGAAACGCCGTTGATG	2	0.10055304172951231	No Hit
GATATACATGGTGGATTCGTCCAACGAGCCCACGAGACACTCGCTAATCT	2	0.10055304172951231	No Hit
CTTTTGGTTCCGTGCCGATTTTGATTTCCTCTGTTGCATGCGTCGTCATC	2	0.10055304172951231	No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG	2	0.10055304172951231	No Hit
CTCCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	2	0.10055304172951231	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTA	2	0.10055304172951231	No Hit
CAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGT	2	0.10055304172951231	No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA	2	0.10055304172951231	No Hit
AGGGCGAGGAGGCCATTGCCAAGCCCATTGCCCAGTCACCATCGGCCGAG	2	0.10055304172951231	No Hit
CAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAAT	2	0.10055304172951231	No Hit
CAGATATACATGGTGGATTCGTCCAACGAGCCCACGAGACACTCGCTAAT	2	0.10055304172951231	No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAAC	2	0.10055304172951231	No Hit
CCCCTGTGTAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
CGTACAAATCGAGCAACTCGTCCACGTAAAACGTCGCTTACGCAGAGAAT	2	0.10055304172951231	No Hit
CCCCGATGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
TACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCT	2	0.10055304172951231	No Hit
CACCCGGTGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATG	2	0.10055304172951231	No Hit
CACCCCGTTTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTAT	2	0.10055304172951231	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.10055304172951231	No Hit
CATCTCCGAGCCCACGAGCACTCGCTAATCTCGTATGCCGTCTTCTGCTT	2	0.10055304172951231	RNA PCR Primer, Index 29 (96% over 27bp)
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA	2	0.10055304172951231	No Hit
GTGTGTGAACCGATCATGTAGTAGGCAAGCTGCGGAGGGACGCAGTGAGG	2	0.10055304172951231	No Hit
TATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTA	2	0.10055304172951231	No Hit
TGAGACATTCCCCTCTGATCGTTGGCCACATCGTGAAACTAGACTTGGAG	2	0.10055304172951231	No Hit
GAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAAT	2	0.10055304172951231	No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA	2	0.10055304172951231	No Hit
GACCAGACCCAATCGTAGGTCAACCATTAGAAGAAATCGTCCGGTGGTAA	2	0.10055304172951231	No Hit
CGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAA	2	0.10055304172951231	Illumina PCR Primer Index 10 (96% over 28bp)
CATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCG	2	0.10055304172951231	No Hit
TCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTG	2	0.10055304172951231	No Hit
GTAGTATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGA	2	0.10055304172951231	No Hit
CCCCTGGTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG	2	0.10055304172951231	No Hit
GGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA	2	0.10055304172951231	Illumina PCR Primer Index 10 (96% over 28bp)
GTCCAGTAGTCCGGAATTCTGGCGAAGATCGAGCCCACGAGACACTCGCT	2	0.10055304172951231	No Hit
TTTCGGAAGAGCACCCCGCGTCAGATCGGAAGAGCCCACGAGACACTCGC	2	0.10055304172951231	No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT	2	0.10055304172951231	No Hit
GCAGAACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCAT	2	0.10055304172951231	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG	2	0.10055304172951231	Illumina PCR Primer Index 10 (96% over 28bp)
CCCCCGTTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.10055304172951231	No Hit
CCCGCTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	2	0.10055304172951231	No Hit
CCCCCGCTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
CGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGG	2	0.10055304172951231	No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGC	2	0.10055304172951231	RNA PCR Primer, Index 12 (95% over 23bp)
CTCCTGGAGCGACAAAAGCGGATGGTGCGCATCGTCTGGGCCATCATGTG	2	0.10055304172951231	No Hit
CAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACC	2	0.10055304172951231	No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG	2	0.10055304172951231	No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTA	2	0.10055304172951231	No Hit
TTTCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC	2	0.10055304172951231	Illumina PCR Primer Index 10 (96% over 25bp)
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC	2	0.10055304172951231	No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA	2	0.10055304172951231	No Hit
CCCCTGTGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC	2	0.10055304172951231	No Hit
AACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGC	2	0.10055304172951231	No Hit
CCGCCAGGGCCTCCCCCCGGGTGTGCCGGACCGTGAGCACCACATGGACG	2	0.10055304172951231	No Hit
GTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGA	2	0.10055304172951231	No Hit
CCCCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	2	0.10055304172951231	No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA	2	0.10055304172951231	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers

Summary