FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s86.351000000d85ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s86.351000000d85ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCCCAAGAAACT15.555555555555555No Hit
TGTCGAGGTGTGTGATGCTAAGCATGTGAGCTTGTCTTGGGAGTGTTTTT15.555555555555555No Hit
CCTTGGAAGCCAAAGAGAAGCACCAACGTAGCCAACAGTGCGACCAGTGA15.555555555555555No Hit
GCACCCCGCTCTAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTA15.555555555555555No Hit
GCCAAGGGGGAAACCATTCGAAGAGTACAAAGGCAAAAGCGAGCTTAACT15.555555555555555No Hit
GTGATGCACTGCGTCCGATTGTTTACGTGCGAATGAGGCGACTCGTCGGC15.555555555555555No Hit
CACCCCCCCTCGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTA15.555555555555555No Hit
ATCTGGATCAGGACTACAAGGAATTCGGGCCGGAGGAGATGCGGTACCCC15.555555555555555No Hit
GTTCAGATCGGAAGAGCACACGAGACATGCGCAGATCTCGTATGCCGTCT15.555555555555555No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15.555555555555555No Hit
CACCCCCAGCTAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTAT15.555555555555555No Hit
GCCGAGAAAAGCCTCTAGTGAGTTCTTATGGGCCCGTACCCTAAACCGAC15.555555555555555No Hit
CATCTTCAGTTGCCAACAGGTAATGCTGGGAACTCTGAGGAAACTGCCGC15.555555555555555No Hit
CGTCTCCCGGGGGAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGT15.555555555555555No Hit
CTATGTGCGCGTCTTTTTCCATCACGATGAGCCGGTCGAGTATGAACCGC15.555555555555555No Hit
GAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA15.555555555555555RNA PCR Primer, Index 41 (96% over 31bp)
GCGGTGGAGCATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGG15.555555555555555No Hit
TTAATGTACCTCCCAGATGGATCCTCGAGCCCACGAGACACTCGCTAATC15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)