FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s78.351000000d84a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s78.351000000d84a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA100.7621951219512195Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC70.5335365853658537RNA PCR Primer, Index 12 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC70.5335365853658537RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC70.5335365853658537RNA PCR Primer, Index 47 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC70.5335365853658537Illumina PCR Primer Index 10 (96% over 26bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA50.38109756097560976TruSeq Adapter, Index 3 (96% over 28bp)
GAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAA50.38109756097560976Illumina PCR Primer Index 10 (96% over 29bp)
AACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGC40.3048780487804878No Hit
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA30.22865853658536583Illumina PCR Primer Index 10 (96% over 28bp)
CTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT30.22865853658536583No Hit
GTGTGTGAACCGATCATGTAGTAGGCAAGCTGCGGAGGGACGCAGTGAGG30.22865853658536583No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCA30.22865853658536583No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG20.1524390243902439No Hit
CCCTAAACCTCCTCCACTTCGACGAGTGGAAGGACACGGACGCCGTGGAA20.1524390243902439No Hit
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1524390243902439Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC20.1524390243902439Illumina PCR Primer Index 10 (96% over 25bp)
GCCCAGGCCTCGTCGAGCTGCTTGTGGAACATCTCGATGCCCGTGACGGT20.1524390243902439No Hit
GATTGGCGGGATCCCCTTGAGGGTTGCACCGCCGACCGACCTTGATCTTC20.1524390243902439No Hit
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1524390243902439RNA PCR Primer, Index 17 (96% over 28bp)
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1524390243902439Illumina PCR Primer Index 9 (100% over 27bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT20.1524390243902439No Hit
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.1524390243902439Illumina PCR Primer Index 5 (96% over 28bp)
ATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAG20.1524390243902439No Hit
ATCATGTAGTAGGCAAGCTGCGGAGGGACGCAGTGAGGTAGCTCATCCCC20.1524390243902439No Hit
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA20.1524390243902439TruSeq Adapter, Index 12 (96% over 29bp)
CCCCGGTTTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC20.1524390243902439No Hit
TCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGATGGT20.1524390243902439No Hit
CCCCTGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC20.1524390243902439No Hit
GATCGGAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC20.1524390243902439Illumina PCR Primer Index 7 (95% over 24bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG20.1524390243902439No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT456.761464E-426.38889154-55
ATGCCGT456.761464E-426.38889146-47
GCTTGAA456.761464E-426.38889158-59
CTGCTTG456.761464E-426.38889156-57
TTGAAAA456.761464E-426.38889160-61
GCCGTCT456.761464E-426.38889148-49
GTATGCC500.00125213823.7544-45
GAAAAAA500.00125213823.7562-63
TCGTATG500.00125213823.7542-43
TCTTCTG500.00125213823.7552-53
CGTCTTC500.00125213823.7550-51
GATCGGA550.002183104221.5909110-11
TCGGAAG550.002183104221.5909112-13
TCTCGTA550.002183104221.5909140-41
ACGAGAC550.002183104221.5909124-25
GGAAGAG600.003621364219.79166814-15
AAAAAAA3553.3232154E-58.02816964-65
Produced by FastQC (version 0.11.2)