FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s70.351000000d8363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s70.351000000d8363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689230
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA362410.2856044062563292No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT308450.2430801553758581No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT288780.22757882077951144No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT242830.19136700966094872No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA242380.19101237821365047No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT231090.18211506923587956No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT155930.12288373683824785RNA PCR Primer, Index 41 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT271900.037.5939525
GATCTTT179400.036.193546
CCGATCT1104900.035.118094
TTCCGAT1120850.034.8346142
TCCGATC1139400.034.175643
CGATCTG431150.033.9538655
CTTCCGA1240700.031.6549171
ATCTTTT188950.031.6240147
GATCTGG155800.031.1579136
CGATCTA206200.030.176415
TCTTTTT239500.024.8104728
CGATCTC319650.024.4590765
GATCTGA133450.023.5982556
GATCTAG80100.023.482686
CTTTTTT253300.023.4400259
GATCTCA89600.022.477236
GATCTTG81400.022.1156526
ATCTGGC84750.021.8578837
ATCTGGG80200.021.7949897
GATCTCG109100.021.0284526