FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s7.351000000d7bbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s7.351000000d7bbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTTCTGCAATCCAAATACGCGTTACACTTTATCATCAAATTTCCGGTA15.263157894736842No Hit
GATCGGAAGAGCACACGTCTCCCAGCTCAGATCGGAAGCTGTCTCTTATA15.263157894736842No Hit
CCGCGGAGCCAAGTCCCGTGTTCAAAAAGAACTGAGAAACAAATTTGTAC15.263157894736842No Hit
TTATTCTCCTGCTGATGATGACCACGGTACTCCATGAAGGCGGCGTCGTT15.263157894736842No Hit
GCGCTTACGCGAGCGAAATCGCAAGGAGTGCGACAACTTCAAGGAGATCA15.263157894736842No Hit
GCCAAGATGAAGAGGCAGTTTGCCAAAATCAAAATAGCAGCCGAAAATCT15.263157894736842No Hit
CATTTGGAGCAACAATAGTCGATCTGAGGATGCAGTTAGGCCATCATCTG15.263157894736842No Hit
TAATAATAGTATTCCCATTTTCATAGTTAGGTTAGGGTATCAGCAAACAG15.263157894736842No Hit
GAATATGCATTTTACATTCGAATAAAGTTAGACGGGGAAATAATGTAATT15.263157894736842No Hit
GGCGAGAGGTTCCGTTCCTATTGGCAGCATCCCAGTGGCAGCCGAACCTT15.263157894736842No Hit
CAATTATGGCGTCATTTACATTCTTGCCTTCCAAAATGGCCAATCCATGA15.263157894736842No Hit
GGTCAACTTCACGCGTTAGCTACGTTACCAAGGAAGTAAATCCCCAACAA15.263157894736842No Hit
TCAACACCCAGAGCACGGATATCAGTGATGCGGCACCGGAGATTCGGTAT15.263157894736842No Hit
CTTATATCGTTTCCCACCGAGATAATGCCTGTATTCTTCGATGGTATCTT15.263157894736842No Hit
GGTCTTATCGGAGCAGATGGTCGATGTGGAGCCAAGAGTCTCCACGGCCT15.263157894736842No Hit
AAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAG15.263157894736842No Hit
CTCTTAAGGGAGACGTGTGCTCTTCCGATCTCTTAAGGGAGACGTGTGCT15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCTCCTGCTTC15.263157894736842No Hit
ATTATATAATAGATAGATTCGCTCTCATTCTCTATGCATCTCCTTTCATT15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)