FastQCFastQC Report
Tue 15 May 2018
HG5M3BCX2_l02n01_r3zt14s69.351000000d834a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s69.351000000d834a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399104
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA473660.5639411060989362No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT394470.46965723962936995No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA314460.37439707854552107No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT307510.3661223863878814No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT146000.1738280654698406No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG127920.15230195982809594No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT123290.14678946706696333No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA105460.1255610122222561No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC105190.12523954936145568No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC94260.11222625651498065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT170200.025.7868825
CCGATCT608150.025.6338794
TTCCGAT623500.025.261812
CTTCCGA631200.025.0892241
TCCGATC630100.024.9218223
CGATCTG257950.024.2698045
GATCTTT134250.024.1653376
ATCTTTT135600.022.8737497
GATCTGG110300.019.5078376
GTATAGA30100.017.848081
TATTATA30950.017.035262
GTATATA57500.016.8671571
TATACTA19800.016.3128625
TATACAG28550.015.9717155
GTATTAT34550.015.9620821
CGATCTA131800.015.8931365
ATCTGGG59500.015.7265377
GTGCTCT475550.015.6457991
TCTTCCG478150.015.6261145
TATAATA32850.015.4715852
Produced by FastQC (version 0.11.2)