FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s67.351000000d8306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s67.351000000d8306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC1343.2157427405807537Illumina Paired End PCR Primer 2 (96% over 26bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA701.6798656107511398RNA PCR Primer, Index 42 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.7439404847612191No Hit
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA260.6239500839932804TruSeq Adapter, Index 5 (96% over 28bp)
TCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTT110.2639788816894648Illumina Paired End PCR Primer 2 (96% over 28bp)
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC100.23998080153587714Illumina PCR Primer Index 5 (96% over 25bp)
GATCGGAAGAGCCCACGAGACACTGAGCGAGCTCGTATGCCGTCTTCTGC90.21598272138228944RNA PCR Primer, Index 1 (95% over 23bp)
CGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG90.21598272138228944RNA PCR Primer, Index 5 (96% over 28bp)
GCACCCCTGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA80.1919846412287017No Hit
GATCGGAAGAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGC80.1919846412287017RNA PCR Primer, Index 1 (95% over 23bp)
AAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT70.167986561075114TruSeq Adapter, Index 5 (95% over 23bp)
CCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60.14398848092152627Illumina Paired End PCR Primer 2 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11999040076793857No Hit
AAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT50.11999040076793857Illumina Paired End PCR Primer 2 (95% over 21bp)
CCCCTGCGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC50.11999040076793857No Hit
CCCCTTGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC50.11999040076793857No Hit
AAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC50.11999040076793857No Hit
GCACCCCGGCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA50.11999040076793857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC200.001931040571.256
GAGACGT200.001931040571.258
GGAGACG250.004676856657.07
AGACGTG250.004676856657.09
GCACCCC352.4760817E-454.285711
AGAGATC300.00962060847.58
GCAGATC650.00520641429.230778
GAGATCG1000.001393124823.759
AGAGCCC2352.39379E-914.14893718-19
AAGAGCC2302.9811417E-813.42391216-17
TGCTTGA2751.4460966E-912.95454658-59
CTTCTGC2751.4460966E-912.95454654-55
TCTGCTT2801.893568E-912.72321356-57
TATGCCG2852.4701876E-912.50000146-47
ATCTCGT2701.6934791E-812.31481540-41
TGCCGTC2903.2032403E-912.28448348-49
CCGTCTT2903.2032403E-912.28448350-51
GTCTTCT2903.2032403E-912.28448352-53
CGTATGC2903.2032403E-912.28448344-45
CTTGAAA2903.2032403E-912.28448360-61
Produced by FastQC (version 0.11.2)