FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s66.351000000d82de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s66.351000000d82de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10739604
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT510940.47575310970497614No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT299840.2791909273377305RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG222810.2074657501338038RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT197760.18414086776383934RNA PCR Primer, Index 41 (95% over 24bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT137790.12830082003023577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT402150.033.0121427
GATCTTT424250.031.8636136
TCTTTTT477000.027.6327768
CGATCTT592700.027.2153995
CTTTTTT515200.025.52869
TATGCTT71500.019.4645145
CTAGGAC84300.017.8613243
ATGCTTG75700.017.7571186
CCGATCT2061800.017.734274
TTCCGAT2090000.017.5677932
TATATGC89000.017.5051483
ATATGCT86800.017.4016084
TCCGATC2119900.017.3176883
TAGGACT85950.017.1868574
CTTCCGA2177600.017.1099281
CTTGTCT79050.016.8843529
GTACTAT94050.016.8835621
GTATATA51550.016.6004661
GTATAGT68250.016.5787091
ATCTAGG93050.016.2986161
Produced by FastQC (version 0.11.2)