FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s65.351000000d82b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s65.351000000d82b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC1013.5045107564191533Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA280.9715475364330326Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA120.4163775156141568Illumina PCR Primer Index 10 (96% over 28bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT70.24288688410825815No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA60.2081887578070784No Hit
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT50.17349063150589866No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGC50.17349063150589866RNA PCR Primer, Index 12 (95% over 23bp)
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA50.17349063150589866Illumina PCR Primer Index 10 (96% over 28bp)
GTCTGGTAGGTAAATCCGCTGGGCGTGAAGGTTGAGGCATGATGGCGAGC40.13879250520471895No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGC40.13879250520471895RNA PCR Primer, Index 12 (95% over 23bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT40.13879250520471895No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG40.13879250520471895Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA40.13879250520471895No Hit
TCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT40.13879250520471895Illumina PCR Primer Index 10 (96% over 27bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA40.13879250520471895Illumina PCR Primer Index 10 (96% over 28bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG30.1040943789035392No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT30.1040943789035392No Hit
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.1040943789035392Illumina PCR Primer Index 10 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1040943789035392No Hit
GATCGGAAGAGCACACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC30.1040943789035392RNA PCR Primer, Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAACCTCGTATGCCGTCTTCTGCTTGAAAAA30.1040943789035392RNA PCR Primer, Index 29 (96% over 27bp)
GTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACC30.1040943789035392No Hit
GTATATATCTCTGGTGGCTCTACAGAATTCACTACAGGAAGTTAACATTA30.1040943789035392No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT30.1040943789035392No Hit
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.1040943789035392Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGCTTGAAAAA30.1040943789035392RNA PCR Primer, Index 29 (96% over 27bp)
ACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGG30.1040943789035392No Hit
TTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGC30.1040943789035392No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA30.1040943789035392No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA30.1040943789035392No Hit
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTA30.1040943789035392No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAAGCTCGTATGCCGTCTTCTGC30.1040943789035392RNA PCR Primer, Index 12 (95% over 23bp)
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA30.1040943789035392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG600.003333912231.6608189
Produced by FastQC (version 0.11.2)