FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s64.351000000d829b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s64.351000000d829b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTG18.333333333333332No Hit
ACCTGACTATTCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATA18.333333333333332No Hit
TTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATG18.333333333333332No Hit
GTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGC18.333333333333332No Hit
CAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACT18.333333333333332No Hit
CCTATCCTTGCGCAGCTCGAGAAGCTCTAACTTTGCGACCTTTCGCCATC18.333333333333332No Hit
TGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGG18.333333333333332No Hit
TCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAA18.333333333333332No Hit
CTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGT18.333333333333332No Hit
CAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGT18.333333333333332No Hit
ACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCT18.333333333333332No Hit
CTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)