FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s62.351000000d8258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s62.351000000d8258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC440.3890706516933416RNA PCR Primer, Index 12 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC360.31833053320364313RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGC350.3094880183924308Illumina PCR Primer Index 10 (96% over 26bp)
GATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGC340.3006455035812185RNA PCR Primer, Index 47 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC320.28296047395879387Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA280.24759041471394466Illumina PCR Primer Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT250.22106287028030772No Hit
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA210.18569281103545848Illumina PCR Primer Index 9 (100% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.16800778141303388No Hit
GAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAA190.16800778141303388Illumina PCR Primer Index 10 (96% over 29bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC180.15916526660182156RNA PCR Primer, Index 11 (96% over 27bp)
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA170.15032275179060925TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT130.11495269254576002Illumina Paired End PCR Primer 2 (95% over 22bp)
CCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA130.11495269254576002Illumina PCR Primer Index 10 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11495269254576002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGATC459.2255266E-442.222228
ATCTTTT500.001549032638.07
GATCTTT500.001549032638.06
GCACCCC500.001549032638.01
CGATCTT500.001549032638.05
TTTGGAA300.00387025431.66666432-33
TCTTTTT600.00378907631.6666648
CTTTTTT650.005605678529.230779
CAGATCG1702.8958311E-927.9411759
CCGATCT1000.001554841323.754
TTCCGAT1100.002706678421.590912
TCCGATC1100.002706678421.590913
CTTCCGA1300.007120929718.2692321
GAGCACA1850.002893638815.4054069
AAGAGCA2050.005728989413.9024397
AGAGCAC2200.00914499312.9545468
AAGAGCC3201.5879777E-911.87500116-17
CGGAAGA4809.336873E-910.8854164
GATCGGA4859.094947E-1210.7731961
TCGGAAG4850.010.7731963
Produced by FastQC (version 0.11.2)