FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s61.351000000d823e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s61.351000000d823e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4741
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC310.6538704914574984RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC250.5273149124657245Illumina PCR Primer Index 10 (96% over 25bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC170.35857414047669267RNA PCR Primer, Index 11 (96% over 27bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA120.2531111579835478Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA90.18983336848766083Illumina PCR Primer Index 11 (100% over 29bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA90.18983336848766083TruSeq Adapter, Index 3 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1265555789917739No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT50.1054629824931449Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT50.1054629824931449No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1054629824931449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT458.6783007E-442.222222
TCCGATC458.6783007E-442.222223
CCGATCT458.6783007E-442.222224
CTTCCGA458.6783007E-442.222221
GAAAAAA1100.00899321512.95454662-63
TTCTGCT1100.00899321512.95454654-55
GCTTGAA1100.00899321512.95454658-59
CTGCTTG1100.00899321512.95454656-57
TTGAAAA1100.00899321512.95454660-61
AAAAAAA6802.8020804E-76.636029264-65
Produced by FastQC (version 0.11.2)