FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s6.351000000d7b95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s6.351000000d7b95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11191490
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT446620.399071079900889No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA406490.3632134773832617No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG357490.3194302099184291No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA338330.3023100588036088No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT335930.3001655722339027No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT306880.27420834937975197No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT256660.22933496790865202Illumina PCR Primer Index 10 (95% over 21bp)
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA192140.17168402062638666No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA177740.15881710120815012No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC170760.15258021943458824No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG158800.14189352802888622Illumina PCR Primer Index 10 (96% over 28bp)
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT150240.1342448592636012No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT147700.13197527764399558No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT137780.12311139982254375Illumina PCR Primer Index 10 (95% over 23bp)
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGA132750.11861691338686806No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC113650.10155037443629043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATCA114900.034.8909382
ATCACTG122850.034.8754355
ATCTTTT239500.033.2990237
CATCACT125700.033.253374
GATCTTT256400.032.9198386
CGATCTT359250.031.996825
ACTGTAC168600.028.9616833
CACTGTA168300.028.2231922
CCATCAC147500.027.9199963
TCACTGT177300.026.736091
CCGATCT1304250.026.2143614
TTCCGAT1313000.026.1700152
TCTTTTT305650.025.9524528
CTTCCGA1345750.025.6921791
TCCGATC1340200.025.6281363
CTTTTTT340900.023.2410289
CTGTACT241000.021.8394661
CGATCTG528350.021.8370535
GATCTAG75300.021.4472796
GATCTGG169450.021.2198646
Produced by FastQC (version 0.11.2)