FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s59.351000000d81ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s59.351000000d81ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268739
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT552800.36204692476569283No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT336370.22029979031012317Illumina Paired End PCR Primer 2 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT190550.1247974701774652Illumina Paired End PCR Primer 2 (95% over 24bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG189740.12426697450260955TruSeq Adapter, Index 5 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT324950.033.926916
ATCTTTT328650.032.027417
CGATCTT500500.029.9325185
TCTTTTT425400.024.6986568
CTTTTTT452350.023.0906589
GATCTGG228350.022.5275066
CCGATCT2137450.021.5956674
TCCGATC2209650.021.012563
CGATCTG798150.020.7219165
TTCCGAT2273200.020.4752772
CTTCCGA2297950.020.3813881
CATAGGT99600.019.2190274
TACATAG121050.018.9133262
ATAGGTA99800.018.5617835
GTATATA47500.018.4151021
ACATAGG124900.017.9880623
ATCTGGG98550.017.9296577
TAGGTAT109350.017.8963366
GTATAAG26700.017.6268161
AGGTATG106050.017.5126447
Produced by FastQC (version 0.11.2)