HG5M3BCX2_l02n01_r3zt14s58.351000000d81df.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l02n01_r3zt14s58.351000000d81df.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1323
Sequences flagged as poor quality	0
Sequence length	101
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC	56	4.232804232804233	RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC	22	1.6628873771730914	Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA	9	0.6802721088435374	Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA	7	0.5291005291005291	TruSeq Adapter, Index 3 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC	7	0.5291005291005291	Illumina PCR Primer Index 11 (95% over 23bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.3779289493575208	No Hit
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA	5	0.3779289493575208	Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACAACTCGTATGCCGTCTTCTGC	4	0.30234315948601664	Illumina PCR Primer Index 11 (95% over 23bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA	3	0.22675736961451248	Illumina PCR Primer Index 10 (96% over 28bp)
CCCCTGTGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	3	0.22675736961451248	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA	3	0.22675736961451248	Illumina PCR Primer Index 10 (96% over 28bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA	3	0.22675736961451248	Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	3	0.22675736961451248	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAA	2	0.15117157974300832	No Hit
CCCCCTTGGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	2	0.15117157974300832	No Hit
GATCGGAAGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC	2	0.15117157974300832	Illumina PCR Primer Index 3 (96% over 25bp)
GAAGAGCACACGTCTCCCTATGCAGATCGGAAGAGCACACGTCTCCCTAT	2	0.15117157974300832	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCTAGGGAGACTGCCGGTGATAAGCCGCGAGGAAGGTGTGGATGACGTC	2	0.15117157974300832	No Hit
TCTCCGAGCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA	2	0.15117157974300832	RNA PCR Primer, Index 29 (96% over 28bp)
ACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTT	2	0.15117157974300832	No Hit
AGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG	2	0.15117157974300832	RNA PCR Primer, Index 17 (95% over 24bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA	2	0.15117157974300832	RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT	2	0.15117157974300832	RNA PCR Primer, Index 29 (96% over 27bp)
AAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2	0.15117157974300832	No Hit
CACGTCTCCCAGACGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTC	2	0.15117157974300832	No Hit
CACCCCTGGACAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT	2	0.15117157974300832	No Hit
AAACTCGGCAGAGTAGCCGAACTGCAGTTAAAACTATCTCTTCCTCTTAT	2	0.15117157974300832	No Hit
TCTCCCGTGCGAGATCGGAAGAGCACACGTCTCCCGTGCGAGATCGGAAG	2	0.15117157974300832	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCGAGCCCACGAACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA	2	0.15117157974300832	RNA PCR Primer, Index 41 (96% over 29bp)
CCCCAGCACAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC	2	0.15117157974300832	No Hit
AAAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC	2	0.15117157974300832	No Hit
AAACAAGGCGATTTGGCTCCTCTTTTATTGTGTACTTGAAAATGCTACGA	2	0.15117157974300832	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers

Summary