FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s57.351000000d81b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s57.351000000d81b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC302.356637863315004Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA151.178318931657502Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.3927729772191673Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG50.3927729772191673Illumina PCR Primer Index 10 (96% over 28bp)
ACTCCGAGATCTACCACACCTTCAACGGAATCACCATCGACCACCGCACC30.2356637863315004No Hit
CGTCTCCCAGTTTAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGT20.15710919088766695No Hit
GTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACC20.15710919088766695No Hit
GTCTGAAGGATCGCAAGGTCTCCGGCAAGCTAATCCGTAAGTGCGCCCCC20.15710919088766695No Hit
GGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.15710919088766695Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCAACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT20.15710919088766695TruSeq Adapter, Index 10 (96% over 26bp)
CCCCTCGGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC20.15710919088766695No Hit
GCACTACACCGGCCTCGCATATCAAGTTCCACACCATCCTTGACCATACA20.15710919088766695No Hit
CCCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.15710919088766695Illumina PCR Primer Index 10 (96% over 28bp)
CCACTAGCGCCAGCACCTCCATCGCGCGCCGAGCCCACGAGACACTCGCT20.15710919088766695No Hit
GAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT20.15710919088766695Illumina PCR Primer Index 10 (95% over 23bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA20.15710919088766695No Hit
ATCGTAGATGACTCCAGTGGGTCCCTGCTGCATGAACCAGTCGATGGACT20.15710919088766695No Hit
TCTCCGAGCCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTT20.15710919088766695RNA PCR Primer, Index 29 (96% over 27bp)
GATCGGAAGAGCCCACGAGACACTCGCTAAACTCGTATGCCGTCTTCTGC20.15710919088766695RNA PCR Primer, Index 12 (95% over 23bp)
CCGCAGAGCAGCATGGCAGTGGGATTAGCCACGTTCTTGCCCACGGCCTC20.15710919088766695No Hit
TACATCACCTTTGTGGAATGACAAATGGGTTGTTTTTTTCTGTGGTTGAC20.15710919088766695No Hit
GTAGTGGACTCTGTGTTGGTGGTGTTGTCAGTGCAACAGAGTGTTCAACA20.15710919088766695No Hit
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA20.15710919088766695Illumina PCR Primer Index 10 (96% over 28bp)
GGTGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC20.15710919088766695No Hit
TAACATAACAATCCCCACATCAATTTGTGTAAATCATATGTAAATCTCGC20.15710919088766695No Hit
CCCCTTGGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC20.15710919088766695No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC20.15710919088766695No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15710919088766695No Hit
CGTGTGGCATGTCTCACCTTATCTATCAGTCCTTCGATGTCACCCATTCC20.15710919088766695No Hit
CCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.15710919088766695Illumina PCR Primer Index 10 (96% over 28bp)
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA20.15710919088766695No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)