FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s54.351000000d814c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s54.351000000d814c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG106.993006993006993Illumina PCR Primer Index 7 (96% over 27bp)
AAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT21.3986013986013985No Hit
GGGTAGTAGTTGAAGAAGGTACCCCGGGACACGTGGGCGGCCTTGGCGAT10.6993006993006993No Hit
TCCACAGGTTGTGCAGCTAGCCAAGACTGTTCATTAAGGTAGGCCCGTGG10.6993006993006993No Hit
CAACATAACCCCACTTTCGATAGTAAAAACTATAACTATAAAGTTATGAG10.6993006993006993No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATCTGTCT10.6993006993006993No Hit
GAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGCTTGAAAAA10.6993006993006993RNA PCR Primer, Index 41 (96% over 27bp)
TCGCCGGGGAGCGCACCGGACACCGCGCGATGTGCGGTGCTCTTCCGGCC10.6993006993006993No Hit
CCCCGTAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG10.6993006993006993No Hit
TAAATATACACAGAGAACGAAATTTAATGCGTTATCTGGAGTATTTAGTG10.6993006993006993No Hit
GTAATACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCT10.6993006993006993No Hit
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC10.6993006993006993RNA PCR Primer, Index 11 (96% over 27bp)
GTTGTCACCACTGCCGGTGTGGTGGTGCGGCGATCGGGCAGGGGTCGAGC10.6993006993006993No Hit
GAAGAGCACCCCCCCGCAGATCGGAAGAGCCCACGAGACATGCGCAGATC10.6993006993006993No Hit
TGTCTACGCAACTCCACATCCTTTCCCACTGAGTCACCACTTAGGGGCCT10.6993006993006993No Hit
GCACCCCAGCCTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6993006993006993No Hit
TCCCACGACAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
GTGAAGCCCTGGACATAAGGGGCATGATGATTTGACGTCCCCAGTGGAGA10.6993006993006993No Hit
TTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTTACGATTTAGC10.6993006993006993No Hit
GAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAA10.6993006993006993Illumina PCR Primer Index 9 (100% over 27bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTCTCCGGAGGCTTC10.6993006993006993No Hit
AATGTCCTCAGCCGTTGAGGTGTCGTACAGCGTGGCCACCAAGCACGCCC10.6993006993006993No Hit
GATCGGAAGAGCACACGTCCCCAGCCGAGATCGGAAGAGCCCACGAGACC10.6993006993006993No Hit
CCCTCGCGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC10.6993006993006993No Hit
GCGTTATGAGCAACAAATGCGTGAGCAGCAGAATCACCTGCAAAAGGAGG10.6993006993006993No Hit
GTGGGAATATTGCACAATGGGCGGAAGCCTGATGCAGCAACGCCGCGTGC10.6993006993006993No Hit
CCCCACTCGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
GATGAGTTTGAAGAGGGAACACGGCGATCCCTCCAAGACCCTGCTTCCGT10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAGCCCC10.6993006993006993No Hit
GATCTGAAGAGCACCCCCGGCGAGATCGGAAGAGCCCACGAGACATGCGC10.6993006993006993No Hit
GTACTCGAATCAGTGGTAGTGGCAGATGTGGTGGAATCTGTACTTGAATT10.6993006993006993No Hit
GTACCAACAGAACCAGCAGTATCCCCAAGTGGCACGACAGTTTGCCGAGC10.6993006993006993No Hit
CTCACATGGGAGACGTGTGCTCTTCCGATCTCACATGGGAGACGTGTGCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCTGGTCAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCC10.6993006993006993No Hit
CAGCCCTGCCCGGGCCTCGCGCAGGCCATCGAGGCAGGAGACGCGGCGGC10.6993006993006993No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAGCCCCCCGAGA10.6993006993006993No Hit
GGCAATGTCTGCACGCGATGTTTAACGTCTGCTTTCCCGGATCCTGGTTC10.6993006993006993No Hit
GCACACGTCTCCCTGCAGAGATCGGAAGAGCTGTCTCTTATACACATCTC10.6993006993006993No Hit
TCCCTGTTGAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGC10.6993006993006993No Hit
AGGCGAAGATGAGGAGGAAGATGGCCAGGGTGCCCACGAGACAGGAGCCT10.6993006993006993No Hit
CCCCATGGCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
ATATTAATCTGGCCGAGAAGTGCAACGGCATCGATATTATACTCGGTGGT10.6993006993006993No Hit
GAAGAGCACACCCCCTGTGAGATCGGAAGAGCCCACGAGACCGGAGCCTA10.6993006993006993No Hit
GTCTAATACTGGATATGACCTTTCTCCTTATGGGGTTTGGTGGAAAGTTT10.6993006993006993No Hit
CCTCTCCCAGGATAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGT10.6993006993006993No Hit
CCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAA10.6993006993006993Illumina PCR Primer Index 7 (96% over 27bp)
CAACAAGAGGTATAATGGGTCGCCTAATTCGAAAGCATTCCTTCGAAGAC10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTGG10.6993006993006993No Hit
ATCTACAAGAGCCATGTGCCTCTCGTCTCGTTGTTGTTCGTTCTATAACC10.6993006993006993No Hit
GAAGAGCACACGTCTCCCGTGCGAGATCGGAAGAGCACACGTCTCCCGTG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACCCCAGGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6993006993006993No Hit
GGATTACGATGAAAACTAAGTTAGTTAAGTGAGAAAATGGTTAAGTTTAA10.6993006993006993No Hit
GATCGGAAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG10.6993006993006993Illumina PCR Primer Index 10 (96% over 25bp)
GTTGTTCATTTGATTTACCCGCTTCATTTGTTCCTGCTCCTTTTCCTTCG10.6993006993006993No Hit
GATCTGTAGGGGGACGGGGTCGGGGAATGGAGTCTCAGTCTGTTGCCCAG10.6993006993006993No Hit
CCCCGCGCCAGATCGGAAGAGCCCACGAGACTCGACGTCATCTCGTATGC10.6993006993006993No Hit
CTCCAGATCGGAAGAGCCCACGAGACGCGTAGTAAGCTCGTATGCCGTCT10.6993006993006993No Hit
GCACCCCACTCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6993006993006993No Hit
TTTCGGAAGAGCACCCCGCGTGAGATCGGAAGAGCCCACGAGACGCGTAG10.6993006993006993No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTTTGCTTGA10.6993006993006993RNA PCR Primer, Index 7 (96% over 26bp)
AGATCGGAAGAGAGAAGGAAGATCGGAAGAGCCCACGAGACGCGTAGTAA10.6993006993006993No Hit
GTCTTGGAGGCCACCTCCTTGAGGAGCTGGGCTCCGATGTTCTCCAGGTG10.6993006993006993No Hit
CTTATACACATCTCCGAGCTCACGAGACTCGACGTCATCTCGTATGCCGT10.6993006993006993No Hit
GGTTAGGCCGGTGCCATTCGGCCAACAGCCTGCCACCAGCCTTCCATCGT10.6993006993006993No Hit
CGGGAGACGTGTGCTCTTCCGATCTCCCACGGGAGACGTGTGCTCTTCCG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGAGACACTGCCCAGTAGAACGGGGAGGAAGGTGGGGATGACGTCAAG10.6993006993006993No Hit
CACGTCTCCCATGAGAGATCGGAAGAGCACACGTCCCCCTAGCAGATCGG10.6993006993006993No Hit
CACCTGCTGCACACCTCGTTCTCCGGCACTGAAGGCGATGTGTCCATCGA10.6993006993006993No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAGCACCCCACCA10.6993006993006993No Hit
GAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAA10.6993006993006993Illumina PCR Primer Index 5 (96% over 28bp)
CATGTAGACAGTGTCTCTAAATACCCGGCAAACCCGACTTGGAGAGTATT10.6993006993006993No Hit
CCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.6993006993006993Illumina PCR Primer Index 5 (96% over 28bp)
GAGCCCCAAGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA10.6993006993006993No Hit
CCCTGCTCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCC10.6993006993006993No Hit
CGGTCAGCATGGGCCTTGGCCCCATCCAGGCCATCTACCAGGCCCGCTTC10.6993006993006993No Hit
GGGCTAAGTGTGTGGACTGTCTGGTAGGTAAATCCGCTGGGCGTGAAGGT10.6993006993006993No Hit
GCCGTGTACCACAGCAAGGATGCTGCCATCGATCCCAAGGTCTATTTGGA10.6993006993006993No Hit
CACCCCAGTTGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTAT10.6993006993006993No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTAGTACCCCCCCTACGGGT10.6993006993006993No Hit
GCACCCCGCTGTAGATCGGAAGAGCACACGTCTCCCGTCCCAGATCGGAA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCTGTAATCCCAGCTGCTCGGGAGGCTAAGGCAGAAGAAGCGCTGGAA10.6993006993006993No Hit
CCCCTGTGCAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
ATGTCAAAGTGAAGAAATTCAAGTAAGCGCGGGTCAACGGCGGGAGTAAC10.6993006993006993No Hit
CTCAACGTACCATGTTTACTGTCCACGATTCCAGTCTCGGTGGCTACCCC10.6993006993006993No Hit
GAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAA10.6993006993006993No Hit
GGCAGCCAGATCCAGTTCCTTGAACTCGACGGCATTATCGTCGACGTACT10.6993006993006993No Hit
GACGTGTGCGCTTCCGATGTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAA10.6993006993006993No Hit
GGCAGGGTCAGGCCGGGACGAGAGCGCGGAGCCCCTCACCCGCTGCCGCG10.6993006993006993No Hit
ACGGAGGCGTACGAAGGTGGGCTCAGAGCGGTCGGAAATCGCTCGTCGCG10.6993006993006993No Hit
CACATCTCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC10.6993006993006993Illumina PCR Primer Index 7 (95% over 24bp)
CAATAAGACGACCATTAGCTGACCTTGTTGACTGCGTAGTGTCTGTCTCT10.6993006993006993No Hit
CCGATACATAGAGCAACTCACACCAACAGGCACAAAACAAAAGTCACTGT10.6993006993006993No Hit
GCACCCCACCGGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTA10.6993006993006993No Hit
GCAGCGCCATGTCCACCGGGTCCAGCTGAACGAAGCGGGCGACATCGCCA10.6993006993006993No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA10.6993006993006993Illumina PCR Primer Index 7 (96% over 27bp)
CCCACAGGAAGAGAAAGCAAAAGCGATTCCGTTAGTAGTGGCGAGCGAAA10.6993006993006993No Hit
GCCCTTTTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.6993006993006993No Hit
CCCCGTGCCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC10.6993006993006993No Hit
AAAAAAGATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC10.6993006993006993No Hit
GATCTAAATGGGGAGACGTGTGCTCTTCCGATCTAAATGGGGAGACGTGT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTATAGGTAACACGCCCCTGGGAGATCGGAAGAGCCCACGAGACACTCG10.6993006993006993No Hit
GGGTCGTACCTCGCCATTCACGCGCTCAACTCGGTGGGGGCGGGCACGCT10.6993006993006993No Hit
GAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAA10.6993006993006993TruSeq Adapter, Index 12 (96% over 29bp)
CCCCGGTTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCC10.6993006993006993No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC10.6993006993006993RNA PCR Primer, Index 41 (96% over 26bp)
CACGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAA10.6993006993006993TruSeq Adapter, Index 12 (96% over 29bp)
ATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT10.6993006993006993RNA PCR Primer, Index 47 (96% over 27bp)
GCACCCGGGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT10.6993006993006993No Hit
ATTTCGCAGAGCCCTGTGTTTTTACTAAACAGTCGGCCACCCCCTGGTCT10.6993006993006993No Hit
GGACAGATCGGAAGAGCACACGTCTCCCAGGACAGATCGGAAGAGCACAC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCCTGCACAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGC10.6993006993006993No Hit
CTCATTGCCCTCACAGCTCGGTAGAACTGTACGGAAAGTCCCGCAACCCC10.6993006993006993No Hit
TCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTTCTGCTTG10.6993006993006993TruSeq Adapter, Index 7 (96% over 26bp)
TGGTGGGAGATTGGCGGTCGGCCATCGACGCCATCGCCGAAAGCGTGCGG10.6993006993006993No Hit
CCCCCCGTTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
TTCCGCATGAGATCGGAAGAGCACACGTCTTCCGCATGAGATCGGAAGAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGCACACGTCTCCCTGCAGAGATCGGAAGAGCACACGTCTCCCTGCAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATAAGGTTGTCCGCCAAATGGGCATCGTCCAGTACATTGGGTTTGTTG10.6993006993006993No Hit
GTGCTCTTCCGATCTGAACCGGGTGTGCTCTTCCTGTCTCTTATACACAT10.6993006993006993No Hit
GTTCGCACCCTCTATGACTGGCCTTTCAAGACCATTCGTCTATGTCGCGC10.6993006993006993No Hit
CCCCCTATGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGC10.6993006993006993No Hit
CGGAAGAGCACCCCAGGCGAGATCGGAAGAGCCCACGAGACGGAGCTACA10.6993006993006993No Hit
ATCATGAACTGCCCGTAGCACGACAACAGTGAAAAATCAAGGGATGCGTA10.6993006993006993No Hit
CTTCAGGTCCCTCGTCCCAACAATCTGTGTCAATTCCATGTCGCACAAGT10.6993006993006993No Hit
CGTCTCCCTACTAAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGT10.6993006993006993No Hit
TTCCGAAGGTACGGAGGCAATTTGCCGGGTTCCTTCAGGATACTTCTCTC10.6993006993006993No Hit
CGACGACGACAAGCCCTTTTTTTCGCTGTTGTGCGTTGTTTGGCTTGTTC10.6993006993006993No Hit
GAAGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAA10.6993006993006993RNA PCR Primer, Index 41 (96% over 31bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCCC10.6993006993006993No Hit
GCACCCCCGCCGAGATCGGAAGAGCCCACGAGACTCGACGTCATCTCGTA10.6993006993006993No Hit
TATACACATCTCCGAGCCCACAGACTCGACGTCATCTCGTATGCCGTCTT10.6993006993006993No Hit
CACCCCAATGGAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTAT10.6993006993006993No Hit
CCCCAGATCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC10.6993006993006993No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers