FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s53.351000000d813f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s53.351000000d813f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATCATATGTAAATCTCGCCATCTCCTGTGCTTGTCTTGCAGACC13.4482758620689653No Hit
CCCTCTGGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC13.4482758620689653No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCACGGGA13.4482758620689653No Hit
GATCGGAAGAGGCCCAGGCTAGATCGGAAGAGCCCACGAGACACTCGCTA13.4482758620689653No Hit
CCCCCTTAGAGATCGGAAGAGCCCAGTAAGATCGGAAGAGCCCACGAGAC13.4482758620689653No Hit
CTCAACTTGCGCGACTTTGACCACCTGGAGCCAAAAGACTTGATGGCCAT13.4482758620689653No Hit
AGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG13.4482758620689653RNA PCR Primer, Index 17 (95% over 24bp)
CACCCGCGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATG13.4482758620689653No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA13.4482758620689653No Hit
GAGCCCAAGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA13.4482758620689653RNA PCR Primer, Index 41 (96% over 29bp)
CTTAATGAAAGTGAACGGAGGTGAGAACCGCAAGGTGCATCATCGACCGA13.4482758620689653No Hit
ATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTAT13.4482758620689653No Hit
CTCGAGCCTCCACACGCGCCGCCATCGTTTGCGTCGCGCAACATGCCTCC13.4482758620689653No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG13.4482758620689653No Hit
GATCGGAAGAGCCCCCTGACGAGATCGGAAGAGCCCACGAGACGGAGCTA13.4482758620689653No Hit
GCAGGACGGTGCCCGTAGAAGATCGGAAGAGCCCACGAGACACTCGCTAA13.4482758620689653No Hit
CTGGAGGACCATTAACTGTCTGCGACATCCGTCTAGTCAACTGGGAAGAA13.4482758620689653No Hit
CACCCCGGTTCAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTAT13.4482758620689653No Hit
CTCATGCCGTGGCCGTGTTTGCCCAATCCAACGGCACTGTCGTCTACGGA13.4482758620689653No Hit
GGATGCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGGACGTCAT13.4482758620689653No Hit
CACCCCCTGGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT13.4482758620689653No Hit
GCGGTGCCCAACGAGCGACCGGAGCGATCGTAGTGCACTGCCGCCTTCTT13.4482758620689653No Hit
GCCAAAATGGGCACAAAGGGGGCGGGGAGGCGAGCCCACGAGACCCTAAG13.4482758620689653No Hit
GTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGC13.4482758620689653No Hit
GCACCCGCCTAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATG13.4482758620689653No Hit
TTTCGGAAGAGCACACCCGTTAGAGATCGGAAGAGCCCACGAGACCCTAA13.4482758620689653No Hit
GTCACGGGGAATGGGATTGGAAATGGGTAGGGGTATGGTCATGTGTATGG13.4482758620689653No Hit
GGTGAGCACGGCGGCCAGCCACCTCATCATTTGCGGCACCAAGCCGGACA13.4482758620689653No Hit
CACCCCGGCCTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT13.4482758620689653No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)