FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s51.351000000d8108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s51.351000000d8108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGATCGGAAGAGCCCCGGTTGAGATCGGAAGAGCCCACGAGACACTGA13.8461538461538463No Hit
ATCTGTAATAAGCCAAAGAAGGAATAAAGATAACTATCCCCACCCAGATC13.8461538461538463No Hit
CCCCTATGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC13.8461538461538463No Hit
GCGAAGAGGAGCTCGAGTACGACCCTGCCTTTTATCCACAGCGTGTGGGC13.8461538461538463No Hit
AAAAAGATCGGAAGAGCACACGTCTCCCACGTTAGATCGGAAGAGCCCAC13.8461538461538463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTCGGAAGAGCACCCCCTGCAGATCGGAAGAGCCCACGAGACACTGAGC13.8461538461538463No Hit
GGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCAC13.8461538461538463No Hit
GGGCCAAAGGCAGGCGCCAAACCGCTGAGCCACCCAGGGATTCCCCTAGA13.8461538461538463No Hit
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC13.8461538461538463Illumina PCR Primer Index 5 (96% over 25bp)
CGTCTCCCCGCTCAGATCGGAAGAGCACCTGTCTCTTATACACATCTCCG13.8461538461538463No Hit
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCT13.8461538461538463No Hit
CTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTAT13.8461538461538463No Hit
GTGGAGGGCAGAGGGAGAGAGAGACTCTCAAGTGGATTCCGCACTGAGCG13.8461538461538463No Hit
GTCCATATACATCAACTTAACGTCTTCGCATGGACCTGTGTTTTTGATAA13.8461538461538463No Hit
GTGTACAAGGCCTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA13.8461538461538463No Hit
TCCTTTGCGAGCGCCACGACAGGCTGACCAGCAATCCGAACTTCCAGGAC13.8461538461538463No Hit
CCCCTGGCAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC13.8461538461538463No Hit
TCTGAAAGGGGGCTTCCAGAGTTTGGGTGGAGCTTGGATCTGCCACAGGG13.8461538461538463No Hit
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA13.8461538461538463RNA PCR Primer, Index 42 (96% over 28bp)
GAAGAGCACCCCGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCT13.8461538461538463No Hit
CTTCCGATCTGGGGCGGGGGAGCACGTCTGACTGACTAACGCTGAATCTC13.8461538461538463No Hit
GTACATTACGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT13.8461538461538463Illumina Paired End PCR Primer 2 (96% over 27bp)
GTATTGACGGGTGTCCGCCACGGCCTGGGCCGTGTGCTACTCCCCTTTCG13.8461538461538463No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCTCGTG13.8461538461538463No Hit
TTCACATCTCCTGTGTAAATTCAGATCTCACTCCTGGAGACCTGTCTCTT13.8461538461538463No Hit
GGCCAGATCGGAAGAGCACACGTCTCCCAGGCCAGATCGGAAGAGCACAC13.8461538461538463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)