FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s50.351000000d80fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s50.351000000d80fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCAGGAGATCGGAAGAGCACACGTCTCCCTCAGGAGATCGGAAGAGC17.142857142857142Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACATAACCGACCCACACGAACAAGATGCAGAAATACGCAAAAACCACTT17.142857142857142No Hit
CTTATACACATCTCCGAGCCCAGGAGACACTCGCTAATCTCGTATGCCGT17.142857142857142No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGAGCCCCCGAGACCCTA17.142857142857142No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC17.142857142857142RNA PCR Primer, Index 41 (96% over 26bp)
CCTGGAAGTCGTTCTCGAACAGGTTGACGGCCGTCTGCCGCGCCTGCAGC17.142857142857142No Hit
CCCTTGGAGGCCTGCACCGCTTCCCTCTCACCCAGGCCACCAGAAGACAC17.142857142857142No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTCAGAGAACACCCCCCGGAGCACC17.142857142857142No Hit
CCCGGTATAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC17.142857142857142No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAATCGCCCTCGTCCCC17.142857142857142No Hit
GATGCTGGCGATGATCTGTTCGGCTTCGCTCTTCACCTGGGCCGGCAGCA17.142857142857142No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTCGTATCTTCCCCCCCCCCTTCTCT17.142857142857142No Hit
CTCCCGGGTGAGATCGGAAGAGCACACGTCTCCCGGGTGAGATCGGAAGA17.142857142857142Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACCCCAGCTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)