FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s5.351000000d7b6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s5.351000000d7b6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626627
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG478290.4968406898906544No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT392500.4077232866714375No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA273840.2844610059161948No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT236560.2457350845732363Illumina PCR Primer Index 10 (95% over 21bp)
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT216260.2246477400651339No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAT199710.20745584097108988No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA182140.18920438072442194No Hit
GATTAATAGGGATAGTCGGGGGCATCAGTATTCAATCGTCAGAGGTGAAA162360.16865720464706901No Hit
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGC160490.16671467586725858No Hit
ACCATACTCCCCCCAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCGA144650.15026031443827625No Hit
GTATTCAATCGTCAGAGGTGAAATTCTTGGATTGATTGAAGACTAACTAC143920.14950200106433956No Hit
GTGTTAGTATTTTGACCCGTTCGGCACCTTACGAGAAATCAAAGTTTTTG137460.14279144709772176No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG134600.13982052072859996Illumina PCR Primer Index 10 (96% over 28bp)
ATACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTAC133620.13880251099372604No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCT132170.13729627210029016No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT124420.1292456849112363Illumina PCR Primer Index 10 (95% over 23bp)
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGC122350.1270953990426761No Hit
ATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAA121120.12581769294686498No Hit
GTGCCGAACGGGTCAAAATACTAACACCGTCCGATCCCTAGTCGGCATAG117030.12156906048193204No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC113080.11746585797912394No Hit
GTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCACTGATTAATGAAAA111450.11577263770581325No Hit
GGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTT111000.11530518425612628No Hit
GAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGA104020.10805446185875904No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCC103740.10776360193450936No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGA98270.10208144555720296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT147850.040.1579786
CGATCTT224100.039.3386345
ATCTTTT142100.039.041997
CGATCTG328950.037.528365
GATCTGG117650.037.5065356
TTCCGAT873850.037.0815622
CCGATCT882650.036.5235064
CTTCCGA920600.035.4756161
TCTACTT107700.034.885422
TACTTGT114800.033.3898774
CTACTTG114350.033.2305073
ACTTGTG121550.031.61385
TGTGCGC119650.031.440958
ATCTGGG48950.031.4394877
ATCTGGT34900.031.3029737
TTGTGCG120450.031.2321267
CTCTACT121700.030.629011
GATCTGA86300.030.3816576
CGATCTA158600.030.3381735
TCTTTTT183250.030.145258
Produced by FastQC (version 0.11.2)