FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s49.351000000d80d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s49.351000000d80d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGATGTTGAACTGCCCTCGTCTGAGGGATGGGTCTGCTCTGCAGG111.11111111111111No Hit
TTCCTCGGGAAGATCGACTTGGGCACGCCCACCGGGATCTCGGTGATGTC111.11111111111111No Hit
GTACATAGATGTAACCAAAAGCTGAAGAAACAGAAACACCGGCCGCACCT111.11111111111111No Hit
CCCCGGGGTAGATCGGAAGAGCACACGTCTCCCGGTTCAGATCGGAAGAG111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCACGAGACGTGTGCTCTTCCGATCTGCCACGAGACGTGTGCTCTTCCG111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAAGAGCACACGTCTCCCGGTGAAGATCGGAAGAGCACACGTCTCCCGGT111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCATCATGCCCCTTATGTCTTGGGCTTCACGCATGCTACAATGCCCGGAC111.11111111111111No Hit
GTGGTGATGAGCGAGCCAAGCGGCACGTTCTGGCCCTGCTGGTTGAGCAC111.11111111111111No Hit
ATACTATGCCTATGCTATGGTGTATAGTTTGGGTCCGGTGTGGTTGGGTT111.11111111111111No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)