FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s46.351000000d8073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s46.351000000d8073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7942110
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT436260.5492998711929198No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT235760.2968480668235519RNA PCR Primer, Index 47 (95% over 22bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG136040.17128949359804888RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT133390.1679528488021445RNA PCR Primer, Index 47 (95% over 24bp)
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT86970.10950490486784999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT301650.040.1849027
GATCTTT333400.037.5835236
CGATCTT443400.035.051445
TCTTTTT367300.032.9635858
CTTTTTT404900.029.8438519
GATCTGG162250.029.5682056
ATCTGGG79050.027.3998727
CCGATCT1526600.025.7939344
TTCCGAT1552750.025.4910742
TCCGATC1571200.025.1766283
CTTCCGA1601000.024.917821
CGATCTG520100.024.7040795
GATCTGA106400.024.151496
ATCTGAG37600.023.244257
ATCTGAC49000.021.810827
ATCTGTG43050.020.8532717
TCTGAGC28800.019.9562288
ATCTGGT42550.019.3122167
TCTGACC28650.019.2317568
TCTGGGC91400.018.4487828
Produced by FastQC (version 0.11.2)