FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s45.351000000d805a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s45.351000000d805a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC130.951683748169839RNA PCR Primer, Index 11 (96% over 27bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC110.8052708638360175Illumina PCR Primer Index 10 (96% over 25bp)
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC100.7320644216691069RNA PCR Primer, Index 41 (96% over 26bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA70.5124450951683748Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA50.36603221083455345Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA50.36603221083455345RNA PCR Primer, Index 17 (96% over 28bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA40.29282576866764276TruSeq Adapter, Index 3 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.29282576866764276No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA30.21961932650073207TruSeq Adapter, Index 11 (96% over 30bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA30.21961932650073207RNA PCR Primer, Index 11 (96% over 30bp)
AAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT30.21961932650073207No Hit
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.14641288433382138Illumina PCR Primer Index 10 (96% over 28bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.14641288433382138Illumina PCR Primer Index 10 (96% over 28bp)
GCACCCCGTTTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA20.14641288433382138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2200.005578048.63636368-69
Produced by FastQC (version 0.11.2)