FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s35.351000000d7ee2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s35.351000000d7ee2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC44.819277108433735Illumina Paired End PCR Primer 2 (96% over 26bp)
CCCCGTGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC22.4096385542168677No Hit
GCACACGTCTCCCGTGTTAGATCGGAAGAGCCCACGAGACACTGAGCGAT11.2048192771084338No Hit
CGTCTCCCGCTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.2048192771084338No Hit
GGTTAAGGTTGTTTTAAACCCCAAGGCCTCTAATCATTCGCTTTACCTCA11.2048192771084338No Hit
GGTGCCACCCGTATCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTC11.2048192771084338No Hit
GTAGAAGGCAGCCTCAGGGAGACTGTCGTGCTTGCCAGAAAGGATCTCCT11.2048192771084338No Hit
CGTCTCCCCTGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.2048192771084338No Hit
GGGCTGGGGCGCACATCGTTCAGCGCGAAGAGCATCGCGAGGGCGGTCAG11.2048192771084338No Hit
CGTCTCCCAGTTTAGATCGGAAGAGCACACGTCTCCCAGTTTAGATCGGA11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCGTCAGAGATCGGAAGAGCACACGTCTCCCGTC11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCCCCACCAAGATCGGAAGAGCACACGTCTCCCCACCAAGATCGGA11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATATCGCCGGATCACAGGATATTGCCGCCTCCCCGACGCTTTTCGCAGG11.2048192771084338No Hit
ATTTGGTTGTGCCCGAAAGGGAACCTGAACACAGGTGCTGCATGGCTGTC11.2048192771084338No Hit
AGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG11.2048192771084338Illumina Paired End PCR Primer 2 (96% over 25bp)
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT11.2048192771084338No Hit
CACGTCCCCAAACCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCG11.2048192771084338No Hit
CGTCTCCCATGGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.2048192771084338No Hit
GCACCCCGACGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT11.2048192771084338No Hit
GGTGTCTTTGTGGCCCAATTACAATCCAGGTTATTAGCAGTCACTCAGTG11.2048192771084338No Hit
GGCAGAGGGAGAAGCAGACTCCCTGCAGGGAGCGGGATGCAGGACCCCTG11.2048192771084338No Hit
GGCTAGGGAGACGTGTGCTCTTCCGATCCCCGGGGGAGATCGGAAGAGCA11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGATCTCACGGGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC11.2048192771084338No Hit
AGATGTAACGGGGCTAAGTCAGTCACCGAAGCTGCGGATATCCGTAAGGA11.2048192771084338No Hit
TGGAAGCTCCAACGTGCCAGCATCCTTTTGGCAAAGAGGCTTGTGCAGAT11.2048192771084338No Hit
GCACCCCTGCCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA11.2048192771084338No Hit
GCACCCCTGTTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA11.2048192771084338No Hit
CGTCTCCCAGCGGAGATCGGAAGAGCCCACGAGACACACTGAGCGATCTC11.2048192771084338No Hit
CGTCTCCCCTTTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.2048192771084338No Hit
CGGATGGCCTTGCGCATCATGGTGTTCTTGCCCATAAGCACGACGGCCAG11.2048192771084338No Hit
GTCTTAGCTAACCAATCGCCCACTAACTCGCAGGCCTCATCCCCTGAAAA11.2048192771084338No Hit
CCCCCAGTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC11.2048192771084338No Hit
CCCCATATCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC11.2048192771084338No Hit
CCATTGGAGCTGGAGCGGGAGTCACCACGGGAGCCCACGAGACACTGAGC11.2048192771084338No Hit
CCCCTCCAGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC11.2048192771084338No Hit
GCACCCCTGGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA11.2048192771084338No Hit
CCCGCGCTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC11.2048192771084338No Hit
GCACCCCCACGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT11.2048192771084338No Hit
CTGCGATGCGACGGCGGACACCGGCTGACGGAACAGATAGGACGCATGGC11.2048192771084338No Hit
CGTCTCCCGTCTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA11.2048192771084338No Hit
GCATTGGCCCGACGATCACGCCCGGGCCCAGGGTCGGATGGCGCTTGGCG11.2048192771084338No Hit
GTTCAAAAGGCAAGAAGCTGAATCACTTTTGATTGCTTGATTTTGGAAAC11.2048192771084338No Hit
GATCGGAAGAGCACACGTCTCCCGGGTCAGATCGGAAGAGCCCACGAGAC11.2048192771084338No Hit
ACGTGGGGACGTGTCTCTTCCCCCTGAAGATCGGAAGAGACACGTCCCCA11.2048192771084338No Hit
CCCGCCCCAGATCGGAAGAGCACCCCCGCGAGATCGGAAGAGCCCACGAG11.2048192771084338No Hit
ATTCAGTATGCAATGACCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTG11.2048192771084338No Hit
GTTCTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC11.2048192771084338No Hit
GAAGAGCACACGTCTCCTGGGGAGATCGGAAGAGCACACGTCTCCTGGGG11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGACGTCTCCCT11.2048192771084338No Hit
GACTAGGTGATTGACGTTAACTACAGAAGAAGCACCGGCTAACTCCGTGC11.2048192771084338No Hit
GCACCCCTTGTGAGATCGGAAGAGCCCACGAGACGCTGAGCGATCTCGTA11.2048192771084338No Hit
GGTCATGATCCCAGAGTCCTGAGACTGAACCCAGACTGGGGCTCCCTACT11.2048192771084338No Hit
GTTGTTCTACTTATGCGGCCTGGCCAACACTTTCCGTTCGTTTGTTTGTT11.2048192771084338No Hit
CACCCGCGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATG11.2048192771084338No Hit
ACCCACAACCATGGGCTCCCCATCATGCCTCAACCTTCACGCCCAGCGGA11.2048192771084338No Hit
GATCTTTGATGGCGGCCTTATGGATCTCCGCGCGCAGCAGCCCCTGCATC11.2048192771084338No Hit
ATATTATATGGCGTTATTACGCGATCAATCGATCGATCGTTTGCTCTAGT11.2048192771084338No Hit
ATAAGGAGAAAGGCAAAGTCTTGAGGATACAAAGAGGAGAAAGACCATTA11.2048192771084338No Hit
GATGATAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT11.2048192771084338Illumina Paired End PCR Primer 2 (96% over 27bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA11.2048192771084338TruSeq Adapter, Index 5 (96% over 28bp)
CATGTGGAGAATCCACCCTGCGCAGATTCTACCCATCCTGGAGGCTTTTG11.2048192771084338No Hit
CACCCCTGTGGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT11.2048192771084338No Hit
CCCTGGCAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC11.2048192771084338No Hit
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA11.2048192771084338RNA PCR Primer, Index 42 (96% over 28bp)
CTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGGGCCCCCCCCTGTAGATCG11.2048192771084338No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAACCCCCCGAAAAACGAG11.2048192771084338No Hit
CCCAAGCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC11.2048192771084338No Hit
GCCCAGGGAGACGTGTGCCCTTCATAGATCGGAAGAGCACACGTCTCCCT11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCAAAAGAAACC11.2048192771084338No Hit
GCCTCAAGCAGGAGGAAATGTGGCAGCGCTACCTCAAGCGCCTCGAGGAG11.2048192771084338No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGT11.2048192771084338No Hit
GCACCCCGTTTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT11.2048192771084338No Hit
CATGAACGTGGTCCTCCTTTCCGTGGTGGAGGGCGATGAAAGGCCCCCCA11.2048192771084338No Hit
GCACACGTCTCCCGGTCGAGATCGGAAGAGCACACGTCTCCCGGTCGAGA11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTTGGATCCCTTCGCTCGCGCAGGTGAGGAACCCCTCGGGACCCCAACA11.2048192771084338No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAGGCACC11.2048192771084338No Hit
CCCCGCGGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC11.2048192771084338No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGAGCCCCAGAAACC11.2048192771084338No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCCCCCGAGACAC11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG50.094.999995
CCTGCAC50.094.999993
CACCGTC50.094.999997
ACCGTCG50.094.999998
CCGTCGT50.094.999999
TTCCTGC50.094.999991
GCACCGT50.094.999996
CTGCACC50.094.999994
TCCTGCA50.094.999992
Produced by FastQC (version 0.11.2)