FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s34.351000000d7ec8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s34.351000000d7ec8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC68.21917808219178RNA PCR Primer, Index 41 (96% over 26bp)
CACCCCGGGAGAGATCGGGAGAGCCCACGAGACCCTAAGACATCTCGTAT11.36986301369863No Hit
TCTTATCAGCGTGTAACTGTTATTGCACTCCTTGTTCTGTCTCTTATACA11.36986301369863No Hit
TTTCACTTCCGTTTTGTTGTTGCCGTCGACGGACCCGGCTCAGATCGGAA11.36986301369863No Hit
GATCCAGCCATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCT11.36986301369863No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAA11.36986301369863No Hit
CGTCTCCCAGTTGAGATCGGAAGAGCACACGTCTCCCAGTTGAGATCGGA11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGGGAACACAA11.36986301369863No Hit
GAGCACCCCCGCTCAGACCGGAAGAGCCCACGAGACTCTAAGACATCTCG11.36986301369863No Hit
CCCATGAGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC11.36986301369863No Hit
CCCCAGGGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC11.36986301369863No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGAGCCCCCCTGGAC11.36986301369863No Hit
AAAAAAGAAAGATCGGAAGAGCACCCCATGTGAGATCGGAAGAGCCCACG11.36986301369863No Hit
GCACCCCTTGAGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTA11.36986301369863No Hit
GCCCAGACGGGAGGGGATGGCTCCGCGGCCCATCAGGTAGACGGCGGGGT11.36986301369863No Hit
GGGTAGAAACGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCCGTGACGA11.36986301369863No Hit
TTTCGGAAGAGCACCCCTCTGCAGATCGGAAGAGCCCACGAGACCCTAAG11.36986301369863No Hit
CACGCGTCTCCCTCGGAAGATCGGAAGAGCACACGTCTCCCTCGGAAGAT11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATG11.36986301369863No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAGCCCCCGAGACCCTA11.36986301369863No Hit
CCCCTGGGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC11.36986301369863No Hit
AAAAAAAGATCGGAAGAGCACACGTCTCCCAGTGCAGATCGGAAGAGCAC11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCAACATGGTGTGCTTCTGTGAACTGATGTTTCTGGCCACTTTTACCGAG11.36986301369863No Hit
CGCCCTCTTGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATG11.36986301369863No Hit
CACCCCTGTAGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTAT11.36986301369863No Hit
CTCCAGCTCCGCCGCCCTCGAGGTGGCGGCCTTCAGGGCCCTCCGCACCC11.36986301369863No Hit
GAGCACACGTCTCCCTCTCAAGATCGGAAGAGCACACGTCTCCCTCTCAA11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCCCTCGCAAGATCGGAAGAGCACACGTCTCCCGGAGTAGATCGGA11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGTCCTGGGACCCAAGTTCATGAAGGATCGCAAAAACCCTT11.36986301369863No Hit
CACCCCTGACCAGATCGGAAGAGCACACGAGACCCTAAGACATCTCGTAT11.36986301369863No Hit
GTCTTAATTGCGATCTATATCGCGTCTATCTTCTTGCGAATCGAGCCGTA11.36986301369863No Hit
GGCCGGGTCAAGGAGGTGGTGTTGCGCGCCGGGGACACCCGGATCCAGGG11.36986301369863No Hit
GAGAGACGTCTCCCTCGCCAGATCGGAAGAGCACACGAGACCCTAAGACA11.36986301369863No Hit
GGGCCCCGTGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT11.36986301369863No Hit
TTTTCATATTTTTGACGAACTTATAAGTGAATTGTTGGCAGCTGGTGCAA11.36986301369863No Hit
TCCCCTACCCATCGTCGCCTTGGTGAGCCGTTACCTCACCAACTAGCTAA11.36986301369863No Hit
TCGGTGTCCTTCGTCTATGTTTGTGATATAATTTCATCAAGCAGCGATAT11.36986301369863No Hit
TCTCCCAGCTGAGATCGGAAGAGCACACGTCTCCCAGCTGAGATCGGAAG11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTGTACCGCGAGGCGGTGGCGTTGCGCGACCTCTGGGCCGCGCAACGC11.36986301369863No Hit
CTCCCGGCACGGGCGTCGCCATCTAACAAATCTTGGAACGAGCATCCGCC11.36986301369863No Hit
TTTTTTTTTTTTTTCGGAAGAGCACACCCGCCTCAGATCCCCTGACGAGA11.36986301369863No Hit
GATCGGAAGAGCACACGTCTCCCTTTTGAGATCGGAAGAGCCCACGAGAC11.36986301369863No Hit
CACCCCTTTTCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTAT11.36986301369863No Hit
TCCCAGGAGAGATCGGAAGAGCACACGTCTCCCAGGAGAGATCGGAAGAG11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAACAGGGGCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT11.36986301369863Illumina PCR Primer Index 10 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCGAAGAGCACCCCCCCC11.36986301369863No Hit
GGCACACCCCTGGCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTC11.36986301369863No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAGCCCCCGA11.36986301369863No Hit
AAAAAGATCGGAAGAGCACACGTCTCCCCTGAAAGATCGGAAGAGCCCAC11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TACTTAAATGTCTGCCATGGTGGGGTAGCTGCACAGATCATTCCATTGCC11.36986301369863No Hit
CCCCGGCTCAGATCGGAAGAGCACACGTCTCCCGGGCCAGATCGGAAGAG11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCATCGAACATCATTGTTTGCGCGCGGCATGCGGTCCTCATCGAGGAGC11.36986301369863No Hit
CCGTAGGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG11.36986301369863No Hit
ACCGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCCAGCAGCCGCGGT11.36986301369863No Hit
GATCGGAAGAGCACCCTTGCGAGATCGGAAGAGCCCACGAGACCCTAAGA11.36986301369863No Hit
CCCCTGGATAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC11.36986301369863No Hit
CGTCTCCCTGGGCAGATCGGAAGAGCACACGTCTCCCTGCCGAGATCGGA11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCCGCAGTCATCGAGTACCCCCGGAGAGGGCTTTACACCCTCTGCTTCG11.36986301369863No Hit
GTTTAGTAAACCTTTACATGAATTTTGGCTAAATAAAAATCCTGTCTCTT11.36986301369863No Hit
CCCCCGCGCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC11.36986301369863No Hit
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA11.36986301369863Illumina PCR Primer Index 10 (96% over 28bp)
CCGCCAATCACGGTCATCACCAGGATCATTTCGCTGACGTGGTAATCGAT11.36986301369863No Hit
CAGCATATATGCTCAAGGTACGTTCCAGTTAGAGGCATAAATAATATAAA11.36986301369863No Hit
GAGCACCCCTGGCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCG11.36986301369863No Hit
TCCCTGGTCAGATCGGAAGAGCACACGTCTCCCTGGCCAGATCGGAAGAG11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGAAGATCGGAAGAGCACACGTCTCCCAGAGAAGATCGGAAGAGCACAC11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCCCCTGCTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATG11.36986301369863No Hit
CCCTTGATCCAGGTTATTCACACACCGCCGATGCGCCATTTTTCACACCT11.36986301369863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA50.094.999996
GTGCAGA50.094.999998
CCCGTGC50.094.999995
CCCCGTG50.094.999994
GGCCCCG50.094.999992
CGTGCAG50.094.999997
TGCAGAT50.094.999999
GCCCCGT50.094.999993
GGGCCCC50.094.999991
AAAAAAA450.010.55555566-67
Produced by FastQC (version 0.11.2)