FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s33.351000000d7ebb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s33.351000000d7ebb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACCCCGCACGAGATCGGAAGAGCCCACGAGACACTCGCTAATC11.3888888888888888No Hit
GCCCACCAAGGCGATGATGGGTAGCCGGGTTGAGAGACCGACCGGCCAGA11.3888888888888888No Hit
GCGTATCTTCTTCAGACCGCGATCCTTCATCACCTGTTTGGCCACATCGC11.3888888888888888No Hit
GAATGACTTCGTTGTGGATCTAAATGTCGTTCGGCCCCGAGGTTGTTCTG11.3888888888888888No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAAGTATAGTAC11.3888888888888888No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT11.3888888888888888No Hit
GATATGGACTCTGGACCGCATTCGCTGGGGAAATAGCCCACTTGCTCGAG11.3888888888888888No Hit
CCATAGCAGATCGGAAGAGCACACGTCTCCCAAGGTAGATCGGAAGAGCA11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCCTGGAGAGATCGGAAGAGCACACGTCTCCCTGGAGAGATCGGAAG11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCGTTGTTGAGGGCAGCAAGTTCGAGAATCAAGTCTCGGACTACACGCAG11.3888888888888888No Hit
CCCCGGTGGAGATCGGAAGAGCACACGAGACACTCGCTAATCTCGTATGC11.3888888888888888No Hit
GTGGAACAGGATGCGGCTCTCGTCGGCCGAGAAGCTGGCGCCGCCGATGT11.3888888888888888No Hit
GCTGTAGCCACCGCATTCGCTGCAGTCGAGGGACACATGGTCGTCCGTCC11.3888888888888888No Hit
ATCTAAACAAATCGTGAGCGGAGAGCATGGGATTCTGCATTTCCTGTGGG11.3888888888888888No Hit
GAACAGCGCCGACTCTATGTAGCTTATCAGCTTCGTTTGACGCACGAGCT11.3888888888888888No Hit
GCACCCCGCCTCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTA11.3888888888888888No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAAGAGCCCCCCTGT11.3888888888888888No Hit
CTGCACCGGGAGAGCACACGTCCCAGTGCAGATCGGAAGAGCCCACGAGA11.3888888888888888No Hit
CATCCGGCTCATAGGGCTCGGCCCTGAATCGCGCCCCATGGGCATAAAAC11.3888888888888888No Hit
GGCCAAGTCCGGTGAGGCCCCCAAGCCAGTTGACCAGCAGAAGAGCACCG11.3888888888888888No Hit
GATCAGCGCCGATACATAGAGCAAGTCACACCAACAGGCACAAAACAAAA11.3888888888888888No Hit
GGACAGCGATGCACATTGGCTATTTCCATGCAGCACGCCAGCGTTTGCCA11.3888888888888888No Hit
CTTCCAACCCGGATCCACGGGCTCCGCTCCCAGGGCCAAAAAAAGCCCCC11.3888888888888888No Hit
GGTCAGATGGCCCAAGGGGTCTTGCAGGCCTTCCAGGAGCAGGGCATCGC11.3888888888888888No Hit
GGGGTTGAAGAGCGAGCGTGCGAGCGAGAGGGCAACATGGGGAAGCGAGA11.3888888888888888No Hit
GTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCA11.3888888888888888No Hit
GCTGAAGTGGGAGCATTAATATCATTGGGTCCCAAGGGATTGGCCACATG11.3888888888888888No Hit
CTTTAGGGATAACAGCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAG11.3888888888888888No Hit
CTTCCGATCTTGGTCGGGAAGAGCCCACGAGACACTCGCTAATCTCGTAT11.3888888888888888No Hit
CGTCTCCCAACCAAGATCGGAAGAGCACACGTCTCCCAACCAAGATCGGA11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGCACACCCTGCGCAGATCGGAAGAGCACCCCAGCTGAGATCGGAAGAGC11.3888888888888888No Hit
ATCTTGGGCGGGGTGTTAGAAGCCCAAAGCGCCAAGGGTGGTATCTCAAG11.3888888888888888No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC11.3888888888888888No Hit
ACCTTTTGGGGCTCGCTGTGCAGAGCCACCTCTGTCTTTAGATCGCGAAC11.3888888888888888No Hit
CTCTGCATCAGCACGGCGATCAGGCCGGTCAGATCCTTTCCCCGGCCCCC11.3888888888888888No Hit
CGTCTCCCCAGTAAGATCGGAAGAGCACACGTCTCCCCAGTAAGATCGGA11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCTTGGTGTGGATGCCAGCGCCGATGCGGTAGATCTTCTTGTTGATCTC11.3888888888888888No Hit
GGCATGCGTCAACGTCGCCAGACTTTTTGCACGCCAGCGACGTCCGACAG11.3888888888888888No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT11.3888888888888888No Hit
GAGCAGGCGCTCTCCCGGGTTGGACGGCATCAATGGCACTATCTGCAAGG11.3888888888888888No Hit
GCCCAAGACAATTGAGACCCTGAGTCGACTTCATGGCATCGAACTGCACG11.3888888888888888No Hit
GCTTCAACTGTACGGCCGCATGGCTATCGAAGCGAGCGTTGGGGGCACGT11.3888888888888888No Hit
CACATGTCTCCCACTGCAGATCGGAAGAGCCCACGAGACACTCGCTAATC11.3888888888888888No Hit
GGCCATCTGCTGGAACAGATTACCAAACTTAAATCTGAAGTGGAGGAGAC11.3888888888888888No Hit
GAGCTATACGCAGTCCAGGCTTCACGACCCACCGCATCCTCCTCACCTCC11.3888888888888888No Hit
CGTGTGCTCTTCCGATCTCGTCCGGGAGACGTGTGCTCTTCCGATCTCGT11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGTGTACCTGAGACGTGATGCGTAGCCATTGTGGTGAAGTTAGTGATC11.3888888888888888No Hit
AAAAAGATCGGAACGTCTCCCCGTTGCAGATCGGAAGAGCCCACGAGACA11.3888888888888888No Hit
GAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT11.3888888888888888Illumina PCR Primer Index 10 (95% over 23bp)
GTAGTTAGTAGTATATATGGATTTTCGTTTCTGCCTGCGTGGTGCATACA11.3888888888888888No Hit
AACCTACACCCCCTAAGCACCCTTCCTGAGGCCGAGCTGGAGGTCCTCCT11.3888888888888888No Hit
CATACAGAGCGCACCAAATGGCGTGGCCGTGGGCGTGGGCATCAAATCGG11.3888888888888888No Hit
CTTCCGATCTGCGGTGGGGGGGCCAACGAACGTCTCGACACCGACAGCGC11.3888888888888888No Hit
CGTCTCCCCCTGCAGATCGGAAGAGCCTACGAGACACTCGCTAATCTCGT11.3888888888888888No Hit
CTGTAGATTATTATGTTTAGGATGTGAAATATATAATGTGCATGGCTAAC11.3888888888888888No Hit
AAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT11.3888888888888888No Hit
GTCGTGGATGTTTCTAGCCGGGCGCGGGCCTTTTCCGCTGTTTGCCCTGG11.3888888888888888No Hit
GTTTTGAGTGTCACGATACATCCAACTGCTTCCGGTGTCACACCAATGGT11.3888888888888888No Hit
AAATTAACACATCAAGGTCGTGTGCTGACTTTAACAGTCCACATCCTTAA11.3888888888888888No Hit
GATCGGAAGAGCACACGTCTCCCACTCAGATCGGAAGAGCACACGTCTCC11.3888888888888888Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCCCAGGCCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGT11.3888888888888888No Hit
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA11.3888888888888888Illumina PCR Primer Index 10 (96% over 28bp)
GGACTAGTGATCTGACGGTGGCTTGTGGATGCGCCGTCACTCAACGGATA11.3888888888888888No Hit
TCATTGTAGTTTTGAGGTTTTTGAAGCCCCTCTTTTTTTCCGACCAAAAA11.3888888888888888No Hit
CGCCCCTCGCGAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTAT11.3888888888888888No Hit
CTCGTGCGCTCGGACACCGAGGGACGCGTTTCATCGAATGGCTTGCGCTC11.3888888888888888No Hit
GTGTGTGCGTGCCTGAAAAATGTCACTTTGACAACTCGCCTCTGGTTGCT11.3888888888888888No Hit
TTATTAAACGACACGGACACTACGCGACCCGTATCCGCCTCTTTAACCAA11.3888888888888888No Hit
CGTCTCCCAGCCCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGT11.3888888888888888No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGGCCCCCCGAACCC11.3888888888888888No Hit
CCTTCTCCTCCCGCCCCTCCTCCTTTCGGCCCACGAGACACACGCTAATC11.3888888888888888No Hit
TTAGTCTTATACTCACAATCTGCTTGAAGAGAATATCTTGACGCTCCAGA11.3888888888888888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT50.094.999992
GATCTTT50.094.999991
CCCCCCC100.047.49999648-49
TCTTTTT100.047.4999963
CTTTTTT100.047.4999964
AAAAAAA250.019.082-83