FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s30.351000000d7e6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s30.351000000d7e6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11577426
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1642241.4184845577937617No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1334391.1525791656971076No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1075440.9289111413884227No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT989120.8543522541193526No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT195970.16926905859730826No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA192880.16660007155303777No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT187960.1623504222786654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT308900.035.4124266
CGATCTT420150.032.88665
ATCTTTT328600.032.3498427
CCGATCT1570600.023.5919764
TTCCGAT1596900.023.3493792
TCTTTTT459500.023.0721658
TCCGATC1620200.022.9840773
CTTCCGA1650900.022.7754251
GATCTGG189850.022.1667886
CGATCTG583250.021.7926985
CTTTTTT490100.021.7091569
GATCTGA135600.019.6158896
ATCTGGG89200.017.5723047
CGATCTA259350.017.2338775
GTATATA46450.016.1730541
GATCTAG92950.015.6880376
GATCTTG92450.015.5159956
GTATTAT29150.015.4955021
CTATACT27900.015.4923334
ATCTGAC65050.015.4068827
Produced by FastQC (version 0.11.2)