FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s3.351000000d7b2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s3.351000000d7b2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5375
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC1943.6093023255813956Illumina Paired End PCR Primer 2 (96% over 26bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA1082.0093023255813955RNA PCR Primer, Index 42 (96% over 28bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA330.613953488372093TruSeq Adapter, Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGC170.31627906976744186RNA PCR Primer, Index 1 (95% over 23bp)
GATCGGAAGAGCCCACGAGACACTGAGCGAACTCGTATGCCGTCTTCTGC170.31627906976744186RNA PCR Primer, Index 1 (95% over 23bp)
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC160.29767441860465116Illumina PCR Primer Index 5 (96% over 25bp)
AAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT120.22325581395348837Illumina Paired End PCR Primer 2 (95% over 21bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCG110.20465116279069767No Hit
AGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG110.20465116279069767Illumina Paired End PCR Primer 2 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.18604651162790697No Hit
AGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAA100.18604651162790697Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG90.16744186046511628TruSeq Adapter, Index 5 (96% over 28bp)
AAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT90.16744186046511628TruSeq Adapter, Index 5 (95% over 23bp)
CCCCTTGGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC80.14883720930232558No Hit
ATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT80.14883720930232558RNA PCR Primer, Index 5 (96% over 26bp)
CCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80.14883720930232558Illumina Paired End PCR Primer 2 (96% over 29bp)
CCCCTGGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC70.13023255813953488No Hit
CCCCCAGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC70.13023255813953488No Hit
CCCCGGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC70.13023255813953488No Hit
AAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT60.11162790697674418No Hit
GCACCCCTGTTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA60.11162790697674418No Hit
CCCCTTTGGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC60.11162790697674418No Hit
ACATAGAGCAACTCACACCAACAGGCACAAAACAAAAGTCACTGTGGCGA60.11162790697674418No Hit
AAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC60.11162790697674418No Hit
GCACCCCTGCCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA60.11162790697674418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT301.1899101E-679.166675
GCACCCC504.7293724E-1176.000011
CCCGTGC200.001962712371.255
TGAGATC200.001962712371.258
ATCTTTT406.5728527E-659.3750047
TTCCGAT406.5728527E-659.3750042
GATCTTT406.5728527E-659.3750046
TCCGATC406.5728527E-659.3750043
CTTCCGA406.5728527E-659.3750041
GCCAGAT250.004753135657.0000047
ACCCCTG250.004753135657.0000043
CCAGATC404.918885E-447.5000048
TCTTTTT502.4650324E-547.5000048
CCGATCT502.4650324E-547.5000044
CGTCTCC458.790276E-442.2222251
CTTTTTT607.236365E-539.5833369
GAGATCG651.15984156E-436.5384649
CAGATCG1153.0066367E-628.9130429
AAAAATA600.004467434219.79166872-73
AGATCGG2700.015.83333312-13
Produced by FastQC (version 0.11.2)