FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s29.351000000d7e42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s29.351000000d7e42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC321.4807959278111986RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC261.203146691346599Illumina PCR Primer Index 10 (96% over 25bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC221.018047200370199RNA PCR Primer, Index 11 (96% over 27bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA170.7866728366496992RNA PCR Primer, Index 17 (96% over 28bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA110.5090236001850995Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA80.37019898195279966Illumina PCR Primer Index 10 (96% over 28bp)
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT70.3239241092086997No Hit
GAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA60.2776492364645997TruSeq Adapter, Index 11 (96% over 30bp)
GAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA60.2776492364645997Illumina PCR Primer Index 10 (96% over 28bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT50.23137436372049977No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG40.18509949097639983No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA40.18509949097639983TruSeq Adapter, Index 3 (96% over 28bp)
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA30.13882461823229986RNA PCR Primer, Index 11 (96% over 30bp)
AGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAA30.13882461823229986Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGAG900.002094123515.83333414-15
GATCGGA950.00300627915.010-11
TCGGAAG950.00300627915.012-13
Produced by FastQC (version 0.11.2)