FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s27.351000000d7e0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s27.351000000d7e0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC584.41400304414003Illumina Paired End PCR Primer 2 (96% over 26bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA171.2937595129375952RNA PCR Primer, Index 42 (96% over 28bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA90.684931506849315TruSeq Adapter, Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGC60.45662100456621RNA PCR Primer, Index 1 (95% over 23bp)
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC50.380517503805175Illumina PCR Primer Index 5 (96% over 25bp)
GAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA30.228310502283105Illumina Paired End PCR Primer 2 (96% over 29bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT30.228310502283105No Hit
CCCCTAGAGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC30.228310502283105No Hit
CCCCTGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC30.228310502283105No Hit
GCACCCCGGCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA30.228310502283105No Hit
AGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG30.228310502283105Illumina Paired End PCR Primer 2 (96% over 25bp)
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA30.228310502283105No Hit
GGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA30.228310502283105Illumina Paired End PCR Primer 2 (96% over 29bp)
CCCCTTACGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC30.228310502283105No Hit
CCCCTGTACAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
GCACCCCGTGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit
CCCCCGGTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CCCCTGTAGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CCCCAGGATAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
GCACCCCGTATGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit
CCCCGGAGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
GAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGCTTGAAAAA20.15220700152207RNA PCR Primer, Index 33 (96% over 27bp)
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCG20.15220700152207No Hit
CGTCTCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT20.15220700152207Illumina Paired End PCR Primer 2 (95% over 24bp)
CCCCTCATGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CACCCCGGGCAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT20.15220700152207No Hit
CACCCCTGTGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTAT20.15220700152207No Hit
GCACCCCGGTTTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit
CCCCTGGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCC20.15220700152207No Hit
GCACCCCAGGTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit
GCACCCCTGGCTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit
CCCCAGCACAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CCCCCCTGTAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CCCCCTGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
AAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT20.15220700152207Illumina Paired End PCR Primer 2 (95% over 21bp)
CCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.15220700152207Illumina Paired End PCR Primer 2 (96% over 29bp)
CCCCTTGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
CCCCTCGTAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
TCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTT20.15220700152207Illumina Paired End PCR Primer 2 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCG20.15220700152207No Hit
CCCCTCGCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC20.15220700152207No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA20.15220700152207No Hit
GTGGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC20.15220700152207No Hit
GAAGAGCACCCCGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCT20.15220700152207No Hit
GCACCCCAGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA20.15220700152207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCCC155.21764E-495.01
Produced by FastQC (version 0.11.2)