FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s26.351000000d7de3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s26.351000000d7de3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC565.049594229035167RNA PCR Primer, Index 41 (96% over 26bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA100.9017132551848512Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC70.6311992786293958Illumina PCR Primer Index 10 (96% over 25bp)
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA40.36068530207394045TruSeq Adapter, Index 3 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA40.36068530207394045RNA PCR Primer, Index 17 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2705139765554554No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA30.2705139765554554No Hit
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG30.2705139765554554No Hit
TCTCCGAGCCCACAAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG20.18034265103697023Illumina PCR Primer Index 10 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA20.18034265103697023Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20.18034265103697023No Hit
CGCTACGACCGCCCGGTCCACCTCGGCCCGCAGGTGATCCGGCTGCGCCC20.18034265103697023No Hit
CCCCTTGTCAGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGC20.18034265103697023No Hit
GTTCATTGGCGAGAAGAGCAAGACGTGCATGATAGCCATGATCTCGCCGG20.18034265103697023No Hit
CATCAGAGTCAGAGCCGAAATCAAAATAAAACCGTAACCTGGCGTGGACG20.18034265103697023No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACAACTCGTATGCCGTCTTCTGC20.18034265103697023Illumina PCR Primer Index 11 (95% over 23bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18034265103697023No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT20.18034265103697023No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18034265103697023No Hit
TCTCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA20.18034265103697023Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18034265103697023No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC20.18034265103697023Illumina PCR Primer Index 11 (95% over 23bp)
CCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAA20.18034265103697023Illumina PCR Primer Index 10 (96% over 28bp)
CCCCTAGTAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCC20.18034265103697023No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACAGCTCGTATGCCGTCTTCTGC20.18034265103697023Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)