FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s22.351000000d7d86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s22.351000000d7d86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8272152
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT520550.6292800229009332No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT290130.35073098269954417TruSeq Adapter, Index 12 (95% over 22bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG170060.2055813287763571TruSeq Adapter, Index 12 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT167490.2024745193270143RNA PCR Primer, Index 12 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT297300.039.5263756
ATCTTTT307850.037.5546387
CGATCTT400800.035.6714675
TCTTTTT388850.029.5729688
CTTTTTT419300.027.3800359
CCGATCT1435450.023.5566274
TTCCGAT1445450.023.5020982
TCCGATC1473650.023.0362423
CTTCCGA1502750.022.805151
GATCTGG142750.022.0274436
CGATCTG484500.021.6857035
ATAATAC24050.018.1700023
GATCTGA119650.017.983236
GTATATA26450.017.9729331
ATCTGTG55850.017.4346377
ATCTGAG41150.017.198827
ATCTGGC69000.017.1408547
GTATAAG20950.017.0185241
TAATACT32550.016.6355084
GTCTAGG18950.016.5569111
Produced by FastQC (version 0.11.2)