FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s21.351000000d7d6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s21.351000000d7d6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC131.045016077170418RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC110.8842443729903537Illumina PCR Primer Index 10 (96% over 25bp)
GATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC110.8842443729903537RNA PCR Primer, Index 11 (96% over 27bp)
GAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA80.6430868167202572Illumina PCR Primer Index 11 (100% over 29bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA50.40192926045016075Illumina PCR Primer Index 10 (96% over 28bp)
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA50.40192926045016075RNA PCR Primer, Index 17 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2411575562700965No Hit
CCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAA30.2411575562700965RNA PCR Primer, Index 11 (96% over 30bp)
CCCCTCGCCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.1607717041800643No Hit
GATCGGAAGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC20.1607717041800643Illumina PCR Primer Index 3 (96% over 25bp)
GTCACCGTCCGTGTCGAGGTGTGTGATGCTAAGCATGTGAGCTTGTCTTG20.1607717041800643No Hit
AAAAAAAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20.1607717041800643No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA20.1607717041800643No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA20.1607717041800643TruSeq Adapter, Index 3 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACAACTCGTATGCCGTCTTCTGC20.1607717041800643Illumina PCR Primer Index 11 (95% over 23bp)
AGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.1607717041800643TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAGATCGGAAGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT20.1607717041800643Illumina PCR Primer Index 11 (100% over 21bp)
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAAT20.1607717041800643No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC20.1607717041800643Illumina PCR Primer Index 11 (95% over 23bp)
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAA20.1607717041800643TruSeq Adapter, Index 11 (96% over 30bp)
CCCCGGGTGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC20.1607717041800643No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC20.1607717041800643No Hit
GATCGGAAGAGCCCACGAGACACTCGCTAATATCGTATGCCGTCTTCTGC20.1607717041800643RNA PCR Primer, Index 12 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3105.7534417E-69.19354866-67
Produced by FastQC (version 0.11.2)