FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s2.351000000d7b1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s2.351000000d7b1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830011
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT728330.6156629947343244No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA536880.4538288256874825No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT437110.36949247130877566No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT428920.36256940082304234Illumina PCR Primer Index 10 (95% over 21bp)
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA356490.30134376037351107No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT330660.27950946114927533No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG290050.24518151335615831Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT270380.22855430988187583Illumina PCR Primer Index 10 (95% over 23bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT139710.11809794597824126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT169350.027.0380137
GATCTTT211650.023.04816
CGATCTT309300.022.4364055
CCGATCT1180900.020.1153094
TTCCGAT1205100.019.9044972
CTTCCGA1218750.019.789021
TCCGATC1219100.019.7070873
GTATTAT40100.019.2063451
CTAGGAC88300.018.235683
TAGGACT89050.018.0820944
TCTTTTT254650.018.0557128
CGATCTG513300.017.4430755
ATCTAGG92850.017.0504721
AGGACTA91700.016.9897675
GATCTGG173450.016.7320776
TCTAGGA99650.016.6829812
CTTTTTT277900.016.4767469
TATTATA40300.016.265112
TAATACT71300.016.1216244
ATATAAG52300.016.075132
Produced by FastQC (version 0.11.2)