FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s16.351000000d7cd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s16.351000000d7cd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCT16.25No Hit
ATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGT16.25No Hit
CATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAA16.25No Hit
TCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCC16.25No Hit
GAGAACCAGCTTATCAGAAAAAAAGTTTGAATTATGGCGAGAAATAAAAG16.25No Hit
ATCGCGAGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACCT16.25No Hit
TAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCT16.25No Hit
GATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGG16.25No Hit
GCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTT16.25No Hit
GTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACT16.25No Hit
AACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATACATATC16.25No Hit
CAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTA16.25No Hit
CGCGCATAAATTTGAGCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACG16.25No Hit
CAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGA16.25No Hit
ACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCT16.25No Hit
TGTTACTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCA16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers