FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s14.351000000d7c87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s14.351000000d7c87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7523421
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT255390.3394599345164919No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT161810.2150750303618527TruSeq Adapter, Index 11 (95% over 23bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT148150.19691839656454158No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG94000.12494316083069125No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT88480.11760607308829321RNA PCR Primer, Index 11 (96% over 25bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA87560.11638322513122688No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT86270.11466857962620994No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG85910.11419007390387963TruSeq Adapter, Index 11 (96% over 30bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA85800.11404386382205649No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT83900.11151841695420207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT149100.025.5175297
CGATCTT210250.023.2016125
CCGATCT621500.022.9966624
GATCTTT170400.022.9689626
TTCCGAT632800.022.6612172
TCCGATC637500.022.50893
CTTCCGA642150.022.4913851
TCTTTTT165200.022.1393268
TATGCTT53450.021.4167275
CTTTTTT181100.020.0906549
TAGGACT84300.020.0588554
CTAGGAC85350.019.7564333
ATCTAGG87000.019.7266271
TCTAGGA92550.019.6566262
CTTGTCT52750.019.6298879
AGGACTA86400.019.2414635
GTATAGT63150.019.1969281
GTCCTAG58750.019.0971721
ATGCTTG57800.018.9009556
GTATTAT19800.018.0078121
Produced by FastQC (version 0.11.2)