FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s13.351000000d7c6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s13.351000000d7c6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490683
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT496540.39752830169495135No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA347380.2781112930333754No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT308210.24675191901035354TruSeq Adapter, Index 11 (95% over 23bp)
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT274920.22010005377608252No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT265310.21240631917405958No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA233000.1865390387379137No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT209020.16734072908583142No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT183520.1469255123999224RNA PCR Primer, Index 11 (96% over 25bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG183400.1468294407919887TruSeq Adapter, Index 11 (96% over 30bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG146760.1174955765029022No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA141900.11360467638158779No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT136280.10910532274336No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG129010.10328498449604397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA553450.029.3949931
CCGATCT547750.029.336064
TTCCGAT557750.029.0996382
TCCGATC565550.028.6395073
ATCTTTT147150.026.2106597
TAGGACT143300.022.274364
ATATAAG64750.021.8604282
CTAGGAC144700.021.8290733
TATAAGA63650.021.4173473
TCTAGGA156150.021.201812
ATCTAGG149750.021.1118641
CGATCTT254050.021.0523875
AGGACTA153250.020.5182195
GACTACC156300.019.9051067
TATGCTT80350.019.5670015
GATCTTT216750.019.0871566
CGATCTG318650.018.7818685
GGACTAC175100.018.0663436
GTCTTAC97750.018.0438371
GTATAGT98250.017.9520111
Produced by FastQC (version 0.11.2)