HG5M3BCX2_l02n01_r3zt14s11.351000000d7c2a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HG5M3BCX2_l02n01_r3zt14s11.351000000d7c2a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2493
Sequences flagged as poor quality	0
Sequence length	101
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC	107	4.292017649418371	Illumina Paired End PCR Primer 2 (96% over 26bp)
GAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAA	32	1.2835940633774567	RNA PCR Primer, Index 42 (96% over 28bp)
GAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAA	15	0.601684717208183	TruSeq Adapter, Index 5 (96% over 28bp)
GATCGGAAGAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGC	7	0.2807862013638187	RNA PCR Primer, Index 1 (95% over 23bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	6	0.24067388688327318	No Hit
GATCGGAAGAGCACACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGC	5	0.20056157240272765	Illumina PCR Primer Index 5 (96% over 25bp)
CCCCTGGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	5	0.20056157240272765	No Hit
AGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG	5	0.20056157240272765	Illumina Paired End PCR Primer 2 (96% over 25bp)
CCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	5	0.20056157240272765	Illumina Paired End PCR Primer 2 (96% over 29bp)
AAAAAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT	4	0.1604492579221821	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTT	4	0.1604492579221821	Illumina Paired End PCR Primer 2 (96% over 28bp)
GAGCCCACGAGACACTGAGCGAACTCGTATGCCGTCTTCTGCTTGAAAAA	4	0.1604492579221821	RNA PCR Primer, Index 33 (96% over 27bp)
GCACCCCTGTCGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	4	0.1604492579221821	No Hit
GCACCCCGCCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	4	0.1604492579221821	No Hit
GGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA	4	0.1604492579221821	Illumina Paired End PCR Primer 2 (96% over 29bp)
GCACCCCGGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	3	0.12033694344163659	No Hit
CCCCTACTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	3	0.12033694344163659	No Hit
GAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT	3	0.12033694344163659	Illumina Paired End PCR Primer 2 (95% over 24bp)
CCCCTTGTCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	3	0.12033694344163659	No Hit
GCACCCCTGTAGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	3	0.12033694344163659	No Hit
ATCATGTAGTAGGCAAGCTGCGGAGGGACGCAGTGAGGTAGCTCATCCCC	3	0.12033694344163659	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.12033694344163659	No Hit
CCCCTGCGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	3	0.12033694344163659	No Hit
GTGCCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	3	0.12033694344163659	No Hit
GCACCCCGGTGCAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTA	3	0.12033694344163659	No Hit
GAGCCCACGAGACACTGAGCGACCTCGTATGCCGTCTTCTGCTTGAAAAA	3	0.12033694344163659	RNA PCR Primer, Index 33 (96% over 27bp)
GATCGGAAGAGCCCACGAGACACTGAGCGAGCTCGTATGCCGTCTTCTGC	3	0.12033694344163659	RNA PCR Primer, Index 1 (95% over 23bp)
ATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCT	3	0.12033694344163659	RNA PCR Primer, Index 5 (96% over 26bp)
CCCCTGTTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	3	0.12033694344163659	No Hit
CCCCTGCTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGTATGC	3	0.12033694344163659	No Hit
CGTCTCCCTGGTGAGATCGGAAGAGCCCACGAGACACTGAGCGATCTCGT	3	0.12033694344163659	No Hit
CACTGACGGACTGGATTAATTGTTTGGGGTTCTCTAGGCTAACGGACATG	3	0.12033694344163659	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGAT	25	0.0044454504	57.0	2
CTTCCGA	25	0.0044454504	57.0	1
TCCGATC	30	0.009147126	47.5	3
CCGATCT	30	0.009147126	47.5	4
AGAGCCC	110	4.3974756E-4	15.113635	18-19
TGCTTGA	125	0.0011613602	13.299999	58-59
CTTGAAA	125	0.0011613602	13.299999	60-61
TGAAAAA	125	0.0011613602	13.299999	62-63
TGCCGTC	140	0.002731107	11.875	48-49
CTCGTAT	140	0.002731107	11.875	42-43
TATGCCG	145	0.0035546883	11.465517	46-47
CCCACGA	145	0.0035546883	11.465517	22-23
AGCCCAC	150	0.0045828037	11.083333	20-21
ACTGAGC	150	0.0045828037	11.083333	32-33
ACACTGA	150	0.0045828037	11.083333	30-31
CACGAGA	150	0.0045828037	11.083333	24-25
CGTATGC	150	0.0045828037	11.083333	44-45
CGAGACA	150	0.0045828037	11.083333	26-27
AGATCGG	150	0.0045828037	11.083333	10-11
AGACACT	150	0.0045828037	11.083333	28-29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content