FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s10.351000000d7c1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s10.351000000d7c1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC823.6887089518668468RNA PCR Primer, Index 41 (96% over 26bp)
GATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC220.9896536212325686Illumina PCR Primer Index 10 (96% over 25bp)
GAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAA210.9446693657219973Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCG90.4048582995951417No Hit
GAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAA80.3598740440845704RNA PCR Primer, Index 17 (96% over 28bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA70.3148897885739991No Hit
GAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAA60.2699055330634278TruSeq Adapter, Index 3 (96% over 28bp)
TCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAA50.22492127755285649Illumina PCR Primer Index 10 (96% over 28bp)
GATCGGAAGAGCCCACGAGACCCTAAGACACCTCGTATGCCGTCTTCTGC50.22492127755285649Illumina PCR Primer Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA40.1799370220422852Illumina PCR Primer Index 10 (96% over 28bp)
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAA40.1799370220422852No Hit
GATCGGAAGAGCCCACGAGACCCTAAGACAACTCGTATGCCGTCTTCTGC40.1799370220422852Illumina PCR Primer Index 11 (95% over 23bp)
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGT40.1799370220422852No Hit
AAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT40.1799370220422852RNA PCR Primer, Index 41 (95% over 24bp)
AAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT40.1799370220422852No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT40.1799370220422852No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT40.1799370220422852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1799370220422852No Hit
CCCCCTGTCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC30.1349527665317139No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT30.1349527665317139No Hit
GATCGGAAGAGCACACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC30.1349527665317139Illumina PCR Primer Index 3 (96% over 25bp)
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA30.1349527665317139No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA30.1349527665317139No Hit
CGTCTCCCCTGCGAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGT30.1349527665317139No Hit
TTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCG30.1349527665317139No Hit
CAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTA30.1349527665317139No Hit
TCTCCAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA30.1349527665317139Illumina PCR Primer Index 10 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1349527665317139No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA30.1349527665317139No Hit
GAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT30.1349527665317139RNA PCR Primer, Index 41 (95% over 24bp)
TAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTT30.1349527665317139No Hit
AGATCGGAAGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG30.1349527665317139Illumina PCR Primer Index 10 (95% over 24bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT30.1349527665317139No Hit
AAAAAAAAAAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC30.1349527665317139No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG30.1349527665317139No Hit
CCCCCTGGCAGATCGGAAGAGCCCACGAGACCCTAAGACATCTCGTATGC30.1349527665317139No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA30.1349527665317139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCCG155.7672727E-495.02
ACCCCGT155.7672727E-495.03
GCACCCC200.001808847971.251
TCCGATC250.004382471657.0000043
CCGATCT250.004382471657.0000044
CTTCCGA250.004382471657.0000041
TTCCGAT300.00901823847.52
CTTTTTT300.00901823847.51
AAAAAAC650.006458387218.26923268-69
AAAAAAA7705.2030162E-55.243506466-67
Produced by FastQC (version 0.11.2)