FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l02n01_r3zt14s1.351000000d7b01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l02n01_r3zt14s1.351000000d7b01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14279636
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1187340.8314917831238836No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT975330.6830216120354888No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA800180.5603644238550618No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT757750.5306507812944251No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT611720.4283862697900703No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG397540.27839645212244907No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT358210.2508537332464217No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA318880.22331101437039433No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT315840.2211821085635516Illumina PCR Primer Index 10 (95% over 21bp)
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC311010.21779966940333773No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC279940.19604141169985004No Hit
GAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAG245950.17223828394505294No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT221680.15524205238845024No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA220200.15420561140353997No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG218560.15305712274458538Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT188860.13225827325010245Illumina PCR Primer Index 10 (95% over 23bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG169300.11856044509818038No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA160440.11235580514797436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT197000.026.498027
CGATCTT380500.020.3227865
GATCTTT280600.020.1269386
TATGCTT100900.019.677445
TATAAGA69650.018.8904423
TAGGACT134450.018.688674
ATATAAG75250.018.6208292
CTAGGAC130400.018.5405963
ATGCTTG99850.018.2669756
CCGATCT1422900.018.2631574
TTCCGAT1457150.017.9805742
CTTCCGA1476750.017.8756831
TCCGATC1469100.017.8407823
GTACTAT143200.017.7303961
TCTAGGA150250.017.6401882
TATTATA49000.017.3516392
GTCTTAC93850.017.2252221
GTATTAT48750.016.9705071
AGGACTA139200.016.8907725
ATCTAGG142900.016.8692551
Produced by FastQC (version 0.11.2)