FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s94.352000000d86a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s94.352000000d86a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231.3120365088419852No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.4563605248146035No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3422703936109527No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3422703936109527No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG50.2852253280091272No Hit
GTTTAATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGT20.11409013120365087No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA20.11409013120365087No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT20.11409013120365087No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC20.11409013120365087No Hit
GTGCTATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11409013120365087No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)