FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s92.352000000d8644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s92.352000000d8644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15255234
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA426960.2798777127902463No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT366950.240540394201754No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA327330.2145689800628427No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGAT273150.17905330065733505No Hit
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT268650.1761034933977414No Hit
ACAATGGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCA251470.16484178479333716No Hit
GTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACT248820.16310467607379867No Hit
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC226270.14832286413961268No Hit
GATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTTGCAGACTA224670.14727404378064604No Hit
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG220760.14471098902842133No Hit
GTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTA216750.14208238300376122No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAG212510.13930300905249962No Hit
GTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTG212220.13911291036243692No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA204850.13428178158394685No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG201660.13219069599325714No Hit
CTACAATCCGAACTGAGAACAACTTTATGGGATTTGCTTGACCTCGCGGT200300.1312991986881355No Hit
ACTTTATGGGATTTGCTTGACCTCGCGGTTTCGCTGCCCTTTGTATTGTC193740.1269990352163723No Hit
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC190500.12487517398946486No Hit
GGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAG174530.11440663578152915No Hit
GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGC173600.1137970089478798No Hit
GTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCG167170.10958206213028263No Hit
CTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCG158950.10419374753609155No Hit
ATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTG153960.10092273904156435No Hit
GTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATG152750.10012956864509585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT624350.069.160582
TCCGATC661050.065.234943
CCGATCT666900.064.5418554
CGATCTA130600.062.9121555
CGATCTT103950.061.3145
CGATCTC222900.051.435015
CGATCTG339000.050.7995345
GATCTGC94300.048.9042136
GCTCTTC222200.047.065433
TCTTCCG225150.046.5967255
GATCTTC49900.045.971156
ATCTGCG29250.044.6527677
ATCTGGC41550.044.4651877
CTTCCGA1012800.042.9394871
ATCTGGT32350.042.722867
GATCTGA86950.042.2775766
CTCTTCC252350.041.5928964
GATCTAT36400.041.4920126
GATCTAA40800.041.440846
GATCTCT72900.041.4350976
Produced by FastQC (version 0.11.2)