FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s9.352000000d7c34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s9.352000000d7c34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4738190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT620301.309149696403057No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA543371.1467881195139915No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT376770.7951770612828949No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA328480.6932605066491635No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG130030.27442968728565126No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT127790.2697021436455693No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG120340.25397884002118953No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG116510.2458955846008708No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC112850.23817111597466542No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA103100.2175936380769872No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC89620.18914395581435106No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAAC87480.1846274632296299No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA85520.18049086254455815No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC85130.179667763428651No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT74390.15700088008290086No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT72410.15282206918675695No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT71860.15166128838227255No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT68510.14459107802768567No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT68120.14376797891177856No Hit
GAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATA67220.14186851941353135No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA67220.14186851941353135No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA66990.14138310198620146No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA66790.1409609998754799No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT66640.14064442329243868No Hit
TCTTATATGTGATTAAATACTTGTATTTTTTCATATGTTCCTCCTATTTA65690.13863943826651104No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA62380.13165364833406848No Hit
CAATAACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAAC61350.12947982246385223No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT58400.12325381633070856No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA57640.12164982830996647No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC57490.12133325172692525No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA57140.12059457303316246No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC55920.11801975015776067No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA54010.11398867500036934No Hit
GGTTAGTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTT52700.11122390617514283No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG51750.1092189211492152No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA50760.10712951570114326No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA50650.10689735954024639No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT48740.10286628438285506No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA48400.10214871079462834No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA47540.10033367171852542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT77750.037.9304284
TCCGATC79850.037.4685363
GAGACGT39250.036.7835278
TTCCGAT82700.036.2347262
GGGAGAC42400.036.0665746
AGACGTG40500.032.3651859
CTTCCGA99650.031.4057541
GGAGACG48550.031.3025847
GCTTGTC36600.026.9899258
ACGGTCC36750.026.8800499
ATGCTTG40400.025.97926
GACGGTC39750.024.7316258
CGACGGT40200.024.336647
ATACCTT64100.023.2641126
GTCTTAC47500.023.1950591
ACGACGG42850.023.1638266
ACTATAC68200.023.1182443
TATGCTT48300.023.1058445
CTATACC67750.023.0612664
CTAGGGG5850.022.7302023
Produced by FastQC (version 0.11.2)